Metabolite Identification
Metabolite Identification with the TriVersa NanoMate offers greater than 10 times more sensitivity than LC/MS/MS for detection of low level, potentially active or toxic recirculating metabolites.
- Characterize “hot” radio-labeled LC fractions
- Obtain structural information on trace-level metabolites by infusing collected HPLC fractions
- Low flow rates enable signal averaging for improved signal-to-noise
- Rapidly switch between mass spectrometry platforms to obtain positive ion, precursor ion, neutral loss scanning or MSn analysis as well as accurate mass
- Less analyte required to get the same data quality versus LC/MS/MS
- Development of a nano-electrospray MSn method for the analysis of serotonin and related compounds in urine using a LTQ-orbitrap mass spectrometer
Moriarty M, Lehane M, O'Connell B, Keeley H, Furey A
Talanta. 2012 Feb 15;90:1-11. Epub 2011 Dec 27
- Advances in structure elucidation of small molecules using mass spectrometry
Kind, T.; Fiehn, O.
Bioanal. Rev. 2010; 2(1):23-60
Article - (13)C Isotope-Labeled Metabolomes Allowing for Improved Compound Annotation and Relative Quantification in Liquid Chromatography-Mass Spectrometry-based Metabolomic Research
Giavalisco, P.; Kohl, K.; Hummel, J.; Seiwert, B.; Willmitzer, L.
Anal Chem. 2009 Jul 9. [Epub ahead of print]
Article - Discovering natural products from myxobacteria with emphasis on rare producer strains in combination with improved analytical methods
Garcia, R.O.; Krug, D.; Müller, R. Methods Enzymol. 2009;458:59-91
Journal Abstract - Negative ion 'chip-based' nanospray tandem mass spectrometry for the analysis of flavonoids in glandular trichomes of Lychnophora ericoides Mart. (Asteraceae)
Gobbo-Neto. L.; Gates, P.J.; Lopes, N.P.
Rapid Commun Mass Spectrom. 2008 Dec;22(23):3802-8
Journal Abstract - High-resolution direct infusion-based mass spectrometry in combination with whole 13C metabolome isotope labeling allows unambiguous assignment of chemical sum formulas
Giavalisco, P.; Hummel, J.; Lisec, J.; Inostroza, A.C.; Catchpole, G.; Willmitzer, L. Anal Chem. 2008 Dec 15;80(24):9417-25
Journal Abstract - Rapid metabolite identification with sub parts-permillion mass accuracy from biological matrices by direct infusion nanoelectrospray ionization after clean-up on a ZipTip and LTQ/Orbitrap mass spectrometry; Erve, J..; DeMaio, W.; Talaat, R. Rapid Commun Mass Spectrom. 2008; 22: 3015–3026
REQUEST HARDCOPY - Dual negative precursor ion scan approach for rapid detection of glutathione conjugates using liquid chromatography/tandem mass spectrometry
Mahajan, M.K.; Evans, C.A. Rapid Commun Mass Spectrom. 2008 Apr;22(7):1032-40
Article - Measurement of Total Acid Number (TAN) and TAN Boiling Point Distribution in Petroleum Products by Electrospray Ionization Mass Spectrometry. K. Qian, et. al. Anal Chem. 2008 Jan 8
REQUEST HARDCOPY - Comparison of a two-dimensional liquid chromatography/mass spectrometry approach with a chip-based nanoelectrospray device for structural elucidation of metabolites in a human ADME study using a quadrupole time-of-flight mass spectrometer; Trunzer, M.; Graf, D.; Kiffe, M. Rapid Commun Mass Spectrom. 2007;21(6):937-44
REQUEST HARDCOPY - Dynamic Range and Mass Accuracy of Wide-Scan Direct Infusion Nanoelectrospray FourierTransform Ion Cyclotron Resonance Mass Spectrometry-Based Metabolomics Increased by the Spectral Stitching Method
Southam, A.D.; Payne, T.G.; Cooper, H.J.; Arvanitis, T.N.; Viant, M.R.
Anal. Chem. 2007; 79:4595-4602
Journal Abstract - Quantitation of Cotinine in Nonsmoker Saliva Using Chip-Based Nanoelectrospray Tandem Mass Spectrometry; Tompkins, B.A.; Van Berkel G.J.; Jenkins, R.A.; Counts R.W. J Anal Toxicol. 2006 Apr;30(3):178-86(9)
REQUEST HARDCOPY - The Combination of Liquid Chromatography/Tandem Mass Spectrometry and Chip-Based Infusion for Improved Screening and Characterization of Drug Metabolites
Roland F. Staack, Emmanuel Varesio, and Gerard Hopfgartner
Rapid Commun Mass Spectrom.; 1-3-2005
REQUEST HARDCOPY - Controlled Protein Precipitation in Combination with Chip-Based Nanospray Infusion Mass Spectrometry. An Approach for Metabolomics Profiling of Plasma
Boernsen, K.O.; Gatzek, S.; Imbert, G.;
Anal. Chem. 2005; 77(22):7255-64
Journal Abstract - Identification of a novel glutathione adduct of diclofenac, 4'-hydroxy-2'-glutathion-deschloro-diclofenac, upon incubation with human liver microsomes
Yu, L. J.; Chen, Y.; Deninno, M. P.; O'Connell, T. N.; Hop, C. E.
Drug Metab Dispos. 2005 Apr.;33(4):484-8. [Epub 2005 Jan 7]
Article - Metabolite fingerprinting: detecting biological features by independent component analysis
Scholz, M.; Gatzek, S.; Sterling, A.; Fiehn, O.; Selbig, J.;
Bioinformatics. 2004, 20(15):2447-54 [Epub 2004 Apr 15]
Article - Quantitation of midazolam in human plasma by automated chip-based infusion nanoelectrospray tandem mass spectrometry
Kapron, J.T.; Pace, E.; Van Pelt, C.K.; Henion, J.
Rapid Commun Mass Spectrom. 2003;17(18):2019-26
Journal Abstract
- Webinar
Lipidome Profiling with LESAOn DemandGavin Reid, PhD
High-resolution mass spectrometry, chemical modification and liquid extraction surface analysis for lipidome profiling of Colon Adenocarcinoma cell lines
