Proteomics
Conduct intact protein analysis including protein identification and protein characterization faster and more completely with automated chip-based nanoelectrospray.
- Identification of faint gel spots with high-quality electrospray ionization MS/MS
- Convert low-abundance MS/MS data into high-quality identifications with infusion for improved signal-to-noise
- Achieve 75 fmol detection limits of protein loaded on a gel
- Zero sample-to-sample carryover
“This workflow yielded up to 220% greater peptide coverage compared with gradient HPLC-ESI and provided protein identifications with up to a two-fold higher throughput rate than either HPLC-ESI approach…” P. Wright et al J Am Soc Mass Spectro 2007
“In the automated format of the TriVersa NanoMate, nano-electrospray measurements are robust, user-friendly and easily amenable for different protein samples while using very low amounts of spraying solution.” M. Mann et al Intl J Mass Spec 2007
- A protein epitope signature Tag (PrEST) library allows SILAC-based absolute quantification and multiplexed determination of protein copy numbers in cell lines
Zeiler, M.; Straube, W. L.; Uhlen, M.; Mann, M.
Mol Cell Proteomics. [Epub 2011 Sep 30] - Identification of the growth-hormone-releasing peptide-2 (GHRP-2) in a nutritional supplement
Thomas, A.; Kohler, M.; Mester, J.; Geyer, H.; Schänzer, W.; Petrou, M.; Thevis, M.
Drug Test Anal. 2010 Mar;2(3):144-8. - Nanoelectrospray with ion-trap mass spectrometry for the determination of beta-casomorphins in derived milk products Juan-García, A.; Font, G.; Juan, C.; Picó, Y. Talanta. 2009 Nov 15;80(1):294-306. [Epub 2009 Jul 4]
- Two highly conserved cysteine residues in HPV16 L2 form an intramolecular disulfide bond and are critical for infectivity in human keratinocytes Campos, S.K.; Ozbun, M.A. PLoS One. 2009; 4(2):e4463. [Epub 2009 Feb 13]
- Towards Heat-stable Oxytocin Formulations: Analysis of Degradation Kinetics and Identification of Degradation Products Hawe, A.; Poole, R.; Romeijn, S.; Kasper, P.; van der Heijden, R.; Jiskoot, W. Pharm Res. 2009 Jul;26(7):1679-88. [Epub 2009 Apr 3]
- Detection and structural features of the betaB2-B3-crystallin heterodimer by radical probe mass spectrometry (RP-MS) Diemer, H.; Atmanene, C.; Sanglier, S.; Morrissey, B.; Van Dorsselaer, A.; Downard, K.M. J Mass Spectrom. 2009 May;44(5):803-12
- DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes Butala, M.; Busby, S.J.; Lee, D.J. Nucleic Acids Res. 2009 Apr;37(5):e37. [Epub 2009 Jan 30]
- Proteomics of the TRAP-induced platelet releasate Piersma, S.R.; Broxterman, H.J.; Kapci, M.; de Haas, R.R.; Hoekman, K.; Verheul, H.M.; Jiménez, C.R. J Proteomics. 2009 Feb 15;72(1):91-109. [Epub 2008 Nov 8]
- Computational analysis and experimental validation of gene predictions in Toxoplasma gondii
Dybas, J. M., Madrid-Aliste, C. J., Che, F. Y., Nieves, E., Rykunov, D., Hogue, R., Weiss, L. M., Kim, K., Fiser, A.
PLoS One. 2008 ;3(12):e3899. [Epub 2008 Dec 9] - Technical Brief: a novel strategy for enrichment of trabecular meshwork protease proteome
Picciani, R.; Junk, A. K.; Bhattacharya, S. K.
Mol Vis. 2008 May 14;14:871-7 - An annotated catalogue of salivary gland transcripts in the adult female mosquito, Aedes aegypti
Ribeiro, J. M. C.; Arcà, B.; Lombardo, F.; Calvo, E.; Phan, V. M.; Chandra, P. K.; Wikel, S. K.
BMC Genomics. 2007 Jan 4;8:6 - Global analysis of community-associated methicillin-resistant Staphylococcus aureus exoproteins reveals molecules produced in vitro and during infectionChristopher Burlak, Carl H. Hammer, Mary-Ann Robinson, Adeline R. Whitney, Martin J. McGavin, Barry N. Kreiswirth and Frank R. DeLeo
Cellular Microbiology (2007) 9(5), 1172-1190
Click here to read article. - Resolving Oligomers from Fully Grown Polymers with IMS-MS
Trimpin, et. al. Anal. Chem. 2007, 79, 7965 – 7974
REQUEST HARDCOPY - A Systematic Evaluation of Chip-Based Nanoelectrospray Parameters for Rapid Identification of Proteins from a Complex Mixture
P. Wright - University of Sheffield
Journal of the American Society for Mass Spectrometry; 12-3-2007
REQUEST HARDCOPY - Protein Identification Using Sequential Ion/Ion Reactions and Tandem Mass Spectrometry
D. Hunt - University of Virginia; PNAS; 7-3-2005
REQUEST HARDCOPY - Investigation of the presence of b ions in electron capture dissociation mass spectra
Cooper, H.J.;
J. Am. Soc. Mass. Spectrom. 2005;16:1932–1940
Journal Abstract - Disposable Chromatography for a High-Throughput Nano-ESI/MS and Nano-ESI/MS-MS Platform
Williams, J.G.; Tomer, K.B.
J Am Soc Mass Spectrom. 2004 Sep;15(9):1333-40
Article - Chip-based nanoelectrospray mass spectrometry for protein characterization
S. Zhang & C. K. Van Pelt; Expert Review in Proteomics - Copyright from Future Drugs LTD. All rights are reserved by Future Drugs Ltd.; 11-3-2004
PDF FILE (876.64 Kb) - Hybrid Ion Trap-Fourier Transform-Ion Cyclotron Resonance for High-Performance Analysis
Lester Taylor; American Laboratory; 1-3-2004
REQUEST HARDCOPY - Automated chip-based nanoelectrospray-mass spectrometry for rapid identification of proteins separated by two-dimensional gel electrophoresis
Zhang, S.; Van Pelt, C.K.; Henion, J.D.
Electrophoresis 2003;24:3620-3623
Journal Abstract - Surface-alkylated polystyrene monolithic columns for peptide analysis in capillary liquid chromatography-electrospray ionization mass spectrometry
Huang, X.; Zhang, S.; Schultz, G.A.; Henion, J.
Anal Chem. 2002 May 15;74(10):2336-44
Journal Abstract - Characterization of a fully automated nanoelectrospray system with mass spectrometric detection for proteomic analyses
C. Van Pelt, S. Zhang, J. Henion; Journal of Biomolecular Techniques; 6-3-2002
REQUEST HARDCOPY - Nanoelectrospray peptide mapping revisited: Composite survey spectra allow high dynamic range protein characterization without LCMS on an orbitrap mass spectrometer
M. Mann - Max-Planck-Institute for Biochemistry
International Journal of Mass Spectrometry; 1-3-1997
REQUEST HARDCOPY
- Webinar
Lipidome Profiling with LESAOn DemandGavin Reid, PhD
High-resolution mass spectrometry, chemical modification and liquid extraction surface analysis for lipidome profiling of Colon Adenocarcinoma cell lines
