- Product Note
Lipid Profiling Using the TriVersa NanoMate®
Shotgun Lipidomics
Faster lipids analysis with no sample-to-sample carryover with automated chip-based nanoelectrospray ionization on the TriVersa NanoMate.
- Quantify and identify individual lipid species with long,stable spray times
- Consistent profile response from reproducible chip-based spray with up to 400 analyses per chip
- Faster analysis compared to conventional nanoelectrospray
- No sample-to-sample carryover
- Shotgun lipidomics on a LTQ Orbitrap mass spectrometer by successive switching between acquisition polarity modesSchuhmann, K.; Almeida, R.; Baumert, M.; Herzog, R.; Bornstein, S.R.; Shevchenko, A.
J. Mass. Spectrom. 2012, 47, 96-104 - Comparative Lipidomics Profiling of Human Atherosclerotic PlaquesStegemann, C.; Drozdov, I.; Shalhoub, J.; Humphries, J.; Ladroue, C.; Didangelos, A.; Baumert, M.; Allen, M.; Davies, AH.; Monaco, C.; Smith, A.; Xu, Q.; Mayr, M.
Circ Cardiovasc Genet. 2011 Apr 21. [Epub ahead of print]
Article - Increase of oxidant-related triglycerides and phosphatidylcholines in serum and small intestinal mucosa during development of intestinal polyp formation in Min miceIkeda, K.; Mutoh, M.; Teraoka, N.; Nakanishi, H.; Wakabayashi, K.; Taguchi, R.
Cancer Sci. 2011 Jan.;102(1):79-87 [Epub 2010 Oct 14]
Article - Orm family proteins mediate sphingolipid homeostasisBreslow, D.K.; Collins, S.R.; Bodenmiller, B.; Aebersold, R.; Simons, K.; Shevchenko, A.; Ejsing, C.S.; Weissman, J.S.Nature. 2010 Feb 25;463(7284):1048-53.
Journal Abstract - Determination of ganglioside composition and structure in human brain hemangioma by chip-based nanoelectrospray ionization tandem mass spectrometrySchiopu, C.; Flangea, C.; Capitan, F.; Serb, A.; Vukelić, Z.; Kalanj-Bognar, S.; Sisu, E.; Przybylski, M.; Zamfir, A.D.
Anal Bioanal Chem. 2009 Dec;395(8):2465-77. [Epub 2009 Oct 16]
Article - A simple modification of a silicic acid lipid fractionation protocol to eliminate free fatty acids from glycolipid and phospholipid fractionsDickson, L.; Bull, I.D.; Gates, P.J.; Evershed, R.P.
J Microbiol Methods. 2009 Sep;78(3):249-54. [Epub 2009 May 27]
Journal Abstract - Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi networkKlemm, R.W.; Ejsing, C.S.; Surma, M.A.; Kaiser, H.-J.; Gerl, M.J.; Sampaio, J.L.; de Robillard, Q.; Ferguson, C.; Proszynski, T.J.; Shevchenko, A.; Simons, K.
J. Cell Biol. 2009 May 18;185(4):601-12. [Epub 2009 May 11]
Journal Abstract - High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometryStåhlman, M.; Ejsing, C.S.; Tarasov, K.; Perman, J.;Borén, J.; Ekroos K.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Sep 15;877(26):2664-72. [Epub 2009 Feb 24]
Journal Abstract - Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry
Ejsing, C.S.; Sampaio, J.L.; Surendranath, V.; Duchoslav, E.; Ekroos, K.; Klemm, R.W.; Simons, K.; Shevchenko, A.
Proc Natl Acad Sci U.S.A. 2009 Feb 17;106(7):2136-41. [Epub 2009 Jan 27]
Article - Accumulation of raft lipids in T-cell plasma membrane domains engaged in TCR signaling
Zech, T.; Ejsing, C.S.; Gaus, K.; de Wet, B.; Shevchenko, A.; Simons, K.; Harder, T.
EMBO J. 2009, 28: 466–476.
Article - Microfluidics-based electrospray ionization enhances the intrasource separation of lipid classes and extends identification of individual molecular species through multi-dimensional mass spectrometry: development of an automated high-throughput platform for shotgun lipidomics
Han, X.; Yang, K.; Gross, R.W.
Rapid Commun Mass Spectrom. 2008 Jul;22(13):2115-24
Journal Abstract - Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics
Matyash, V.; Liebisch, G.; Kurzchalia, T.V.; Shevchenko, A.; Schwudke D.;
J. Lipid Res. 2008, 49: 1137-1146
Article - Shotgun Lipidomics Identifies a Paired Rule for the Presence of Isomeric Ether Phospholipid Molecular Species
Yang, K.; Zhao, Z.; Gross, R. W.; Han, X.
PLoS ONE. 2007 ; 2(12): e1368
Article - Detection of the abundance of diacylglycerol and triacylglycerol molecular species in cells using neutral loss mass spectrometry
Murphy, R.C.; James, P.F.; McAnoy, A.M.; Krank, J.; Duchoslav, E.; Barkley, R.M.
Anal. BioChem. 2007 , 366:59-70
Article - Osmolality, temperature and membrane lipid composition modulate the activity of betaine transporter BetP in Corynebacterium glutamicum
Özcan, N.; Ejsing, C.S.; Shevchenko, A.; Lipski, A.; Morbach, S.; Krämer, R.
J. Bacteriol. 2007 , 189(20): 7485-7496
Article - Electrospray mass spectrometry of human hair wax esters
Fitzgerald, M.; Murphy, R.C.;
J. Lipid Res. 2007 , 48:1231–1246
Article - FceRI and Thy-1 domains have unique protein and lipid compositions
Surviladze, Z.; Harrison, K.A.; Murphy, R.C.; Wilson, B.S.;
J. Lipid Res. 2007 , 48:1325–1335
Article - Top-Down Lipidomic Screens by Multivariate Analysis of High-Resolution Survey Mass Spectra
Dominik Schwudke, et.al. - MPI of Molecular Cell Biology and Genetics; Analytical Chemistry; 5-3-2007
REQUEST HARDCOPY - Identification and Structure Determination of Sphingolipids from Sphingoid Bases to Sulfatides using QTrap and Chip-based NanoESI with Hybrid Q-TOF MS/MS M. Cameron Sullards - Georgia Institute of Technology
Keystone Symposia on Lipids; 2-24-2007
PDF FILE (211.89 Kb) - Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning; Ejsing CS, Duchoslav E, Sampaio J, Simons K, Bonner R, Thiele C, Ekroos K, Shevchenko A; Analytical Chemistry; 9-3-2006
REQUEST HARDCOPY - Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry;
C.S. Ejsing - Max Planck Institute of Molecular Cell Biology and Genetics; Journal of Mass Spectrometry; 2-3-2006
REQUEST HARDCOPY - Liver-directed overexpression of mitochondrial glycerol-3-phosphate acyltransferase results in hepatic steatosis, increased triacylglycerol secretion and reduced fatty acid oxidation
D. Linden - AstraZeneca R&D, Molndal; FASEB Journal; 2-3-2006
REQUEST HARDCOPY - Analysis of polyunsaturated aminophospholipid molecular species using
R. Murphy et al - University of Colorado at Denver; Analytical Biochemistry; 2-1-2006
REQUEST HARDCOPY - Lipid Profiling by Multiple Precursor and Neutral Loss Scanning Driven by the Data-Dependent Acquisition
A. Shevchenko - MPI of Molecular Cell Biology and Genetics
Analytical Chemistry; 1-3-2006
REQUEST HARDCOPY - Sphingolipidomics: High-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry
Merrill Jr., A.H., Sullards, M.C., Allegood, J.C., Kelly, S., Wang, E.; Methods; 9-3-2005
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- Webinar
Lipidome Profiling with LESAOn DemandGavin Reid, PhD
High-resolution mass spectrometry, chemical modification and liquid extraction surface analysis for lipidome profiling of Colon Adenocarcinoma cell lines
