RePlay® Select References

Proteomic researchers have demonstrated the benefits of RePlay as follows:

RePlay

Journal Articles

Proteomics characterization of extracellular space components in the human aorta
Didangelos, A.; Yin, X.; Mandal, K.; Baumert, M.; Jahangiri, M.; Mayr, M.
Mol. Cell. Proteomics. 2010 Sep; 9(9):2048-62.

Pressurized pepsin digestion in proteomics: An automatable alternative to trypsin for integrated top-down bottom-up proteomics
López-Ferrer, D.; Petritis, K.; Robinson, E.W.; Hixson, K.K.; Tian, Z.; Lee, J.H.; Tolić, N.; Weitz, K.K.; Belov, M.E.; Smith, R.D.; Paša-Tolić, L.
Mol. Cell. Proteomics. 2010; Sep 10. [Epub ahead of print]

Quantitative proteomic analysis of single pancreatic islets
Waanders, L.F.; Chwalek, K.; Monetti, M.; Kumar, C.; Lammert, E.; Mann, M.
Proc. Natl. Acad. Sci. U.S.A. 2009 Nov 10; 106(45):18902-7.

A novel chromatographic method allows on-line reanalysis of the proteome
Waanders, L.F.; Almeida, R.; Prosser, S.; Cox, J.; Eikel, D.; Allen, M.H.; Schultz, G.A.; Mann, M.
Mol. Cell. Proteomics. 2008 Aug; 7(8):1452-9.

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Application Notes

Increased information gain from complex proteomic samples using RePlay: Dividing a nanoLC flow post-column and exploiting twice the analytical possibilities from one sample injection
Eikel, D. ; Koller, T.*; Chen, E.*; Prosser, S.
Advion Application Note (*Stony Brook University, Stony Brook, NY, USA)

“RePlay” significantly increases the number of protein assignments from complex proteomic samples
Schneider, A.; Lubeck, M.; Baessmann, C.; Almeida, R.
Bruker Daltonics Application Note # LCMS-54

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Oral Presentations

Integrated top-down/bottom-up proteomics for the characterization of protein isoforms
Robinson, E.W.; Paša-Tolić, L.; Tian, Z.; Tolić, N.; López-Ferrer, D.; Zhang, H.; Wu, S.; Smith, R.D.
Presented at 7th Advion Users’ Meeting at 58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City, UT, May 2010.

uTOPIA: Universal tool for on-line processing and iterative analysis
Caldwell Busby, J.; Pascal, B.; Rose, K.; Cavett, V.; Hoopmann, M.; MacCoss, M.
Presented at 7th Advion Users’ Meeting at 58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City, UT, May 2010.

Digests by consecutive, targeted LC-MSMS runs with both CID and ETD
Albers, C., Hartmer, R.; Belau, E.; Almeida, R.; Schneider, A.; Lubeck, M.
Presented at 6th Advion Users’ Meeting at 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA, May 2009.

Integrated “RePlay” top-down/bottom-up proteomics
Petritis, K.; López-Ferrer, D.; Tian, Z.; Robinson, E.W.; Belov, M.; Smith, R.D.; Paša-Tolić, L.
Presented at 6th Advion Users’ Meeting at 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA, May 2009.

Characterization and novel experimental designs using RePlay and LC/MS
Caldwell Busby, J.; Cavett, V.; Pascal, B.; Dorsey, C.; Prater, S.; Cleveland, J.; Prosser, S.; Corso, T.
Presented at 5th Advion Users’ Meeting at 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO, May 2008.

Mining the 24-hour proteome Master, S.; Bierl, C.; Everett, L.; Li, L.; Ryvkin, P.; Prosser, S.; Schultz, G. Presented at 5th Advion Users’ Meeting at 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO, May 2008.

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Poster Presentations

Up two speed: rapid sample characterization by combining RePlay and UPLC-MS for duplicate analysis in half the time
Sprenger, R.R.; Jensen, O.N.
J. Am. Soc. Mass Spectrom. 2010; 21(5 Suppl):S80.

Enabling data-dependent gas-phase fractionation in a single chromatographic-based shotgun proteomics analysis
Almeida, R.; Alpha, C.; Allen, M.
J. Am. Soc. Mass Spectrom. 2010; 21(5 Suppl):S81.

Increased sequence coverage in complex protein digests by consecutive, targeted LC-MSMS runs with both CID and ETD
Schneider, A.; Lubeck, M.; Almeida, R., Clemens, P.
J. Biomol. Tech. 2010 September; 21(3 Suppl):S55–S56.

Combining nanoLC/MS with low volume fractionation in a single LC/MS experiment
Almeida, R.; Waanders, L. F.; Bandilla, P.; Allen, M.; Mann, M.
Presented at 18th International Mass Spectrometry Conference, Bremen, Germany, Aug. - Sept. 2009.

Characterization of protein isoforms using tandem MS of intact and on-line digested proteins from a single intact protein HPLC separation
Robinson, E. W.; Tian, Z.; Tolić, N.; López-Ferrer, D.; Petritis, K.; Adkins, J. N.; Smith, R. D.; Paša-Tolić, L.
J. Am. Soc. Mass Spectrom. 2009; 20(5 Suppl):S39.

Integration of top-down and bottom-up approaches: Application of RePlay for confident protein/peptide identifications by on-line post-column digestion and high-resolution MS
Petritis, K.; López-Ferrer, D.; Tian, Z.; Robinson, E.; Belov, M.; Paša-Tolić, L.; Fjeldsted, J.; Leonard, L.; Prosser, S. J.; Schriemer, D.; Lewis, D. F.; Smith, R. D.
J. Am. Soc. Mass Spectrom. 2009; 20(5 Suppl):S123.

RePlay combined with a scheduled precursor list (SPL) significantly improves the number of protein assignments from complex proteomic samples
Eikel, D.; Albers, C.; Lubeck, M.; Rule, G. S.; Prosser, S. J.
J. Am. Soc. Mass Spectrom. 2009; 20(5 Suppl):S99-100.

A novel approach enabling dual MS-analyses of a single LC-injection with excellent sensitivity
Waanders, L. F.; Almeida, R.; Bandilla, P.; Prosser, S.; Cox, J.; Allen, M.; Schultz, G.; Mann, M.
J. Am. Soc. Mass Spectrom. 2008; 19(5 Suppl):S110.

Combining exploratory & targeted analysis for identification & quantitation of biomarker candidates in a single nanoLC/MS run
Almeida, R.; Waanders, L. F.; Schultz, G. A.; Bandilla, P.; Allen, M.; Mann, M.
J. Am. Soc. Mass Spectrom. 2008; 19(5 Suppl):S53.

Déjà vu LC-MS/MS: How to RePlay an LC-MS/MS chromatogram without injecting the sample again
Eikel, D.; Prosser, S. J.; Schultz, G. A.; Almeida, R.; Allen, M. H.
J. Am. Soc. Mass Spectrom. 2008; 19(5 Suppl):S110.

Improved overall mass spectral acquisition rates using a novel HPLC storage loop
Bierl, C.; Prosser, S. J.; Master, S. R.
J. Am. Soc. Mass Spectrom. 2008; 19(5 Suppl):S126.

Mapping post translational modifications using single aliquot/dual scanning method
Cavett, V.; Prater, S. M.; Prosser, S. J.; Cleveland, J. L.; Coenen, S.; Dorsey, F. C..; Corso, T. N.; Busby, J.
J. Am. Soc. Mass Spectrom. 2008; 19(5 Suppl):S125.

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Recent Publication

Proteomics Characterization of Extracellular Space Components in the Human Aorta

A. Didangelos, X. Yin, K. Mandal, M. Baumert, M. Jahangiri, and M. Mayr
Mol Cell Proteomics. 2010 September; 9(9): 2048–2062.

The authors "expect this proteomics methodology to further our understanding of the composition of the vascular extracellular environment, shed light on ECM remodeling and degradation, and provide insights into important pathological processes, such as plaque rupture, aneurysm formation, and restenosis."

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