TWICE
the analytical possibilities from EVERY nanoLC injection
RePlay (patent pending) provides
proteomic researchers the ability to analyze a single nanoLC injection
twice without the need for additional time or sample. Obtain maximum
information from a single nanoLC/MS run for more coverage and increased
analytical confidence.
- Use Existing Equipment - RePlay is compatible with
existing MS, nanoLC, and nanoelectrospray ionization sources (e.g.,
nanospray needles, pulled capillaries, ESI Chip, etc.).
- Twice the Possibilities - RePlay performs a second
analysis of one sample without a reinjection, thereby allowing for twice
the analytical possibilities from every nanoLC injection. Use the second
analysis to obtain more targeted information.
- No Additional Time - RePlay splits the sample from
one injection, so a second analysis is provided with no additional time
required.
- No Additional Sample - Utilizing the concentration-sensitive
nature of electrospray, RePlay can split the sample to two slower flows
at the same concentration without decrease in overall signal intensity.
How it works
RePlay uses a nanoflow splitter
and the unique RePlay valve to split the nanoLC eluent to a direct on-line
analysis and to a Capture Cartridge. During equilibration of the
column, the RePlay valve switches to direct the captured chromatogram
to the MS for a second analysis of the same injection.
RePlay analysis enables
the following analytical possibilities:
- Orthogonal MS Experiments - CID, ETD, HCD, IRMPD,
ECD, MS3, precursor ion, etc.
- Separate Mass Ranges - Separate the mass ranges into
the direct and RePlay analyses to identify more ions within each mass
range.
- Improved Resolution - Slow down the chromatography
during the RePlay analysis to improve chromatographic resolution with
the use of a refocusing column.
- Vary MS Conditions - Use a different collision energy
during the RePlay analysis to improve MS/MS sensitivity.
- Focused Analysis - Identify peaks of interest in
the first analysis and use the RePlay analysis to dig deeper.
- Exclusion and Inclusion List - Use exclusion and
inclusion lists to look at additional ions or target those without a
positive identification during the RePlay analysis.
- Different Charge States - Look at doubly charged
ions in the first analysis and triply charged and greater ions during
the RePlay analysis to enhance ion fragmentation and improve structural
information.
- Extended Analysis - Slow down the chromatography
without a refocusing column to broaden peak widths and provide more
time during the RePlay analysis for data mining.
- Positive and Negative Ion Analysis - Analyze in negative
ion mode to identify PTMS, then switch to positive ion mode during the
RePlay analysis to increase structural information.
- Exploit Random Gains - Improve coverage from the
random gains that generally occur during a repeat analysis of the same
sample.
- Reproducibility -
Increase analytical completeness with a duplicate run for higher confidence.
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