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RePlay™ for nanoLC/MS

TWICE the analytical possibilities from EVERY nanoLC injection

RePlay (patent pending) provides proteomic researchers the ability to analyze a single nanoLC injection twice without the need for additional time or sample.  Obtain maximum information from a single nanoLC/MS run for more coverage and increased analytical confidence.

  • Use Existing Equipment - RePlay is compatible with existing MS, nanoLC, and nanoelectrospray ionization sources (e.g., nanospray needles, pulled capillaries, ESI Chip, etc.).

  • Twice the Possibilities - RePlay performs a second analysis of one sample without a reinjection, thereby allowing for twice the analytical possibilities from every nanoLC injection. Use the second analysis to obtain more targeted information.

  • No Additional Time - RePlay splits the sample from one injection, so a second analysis is provided with no additional time required.

  • No Additional Sample - Utilizing the concentration-sensitive nature of electrospray, RePlay can split the sample to two slower flows at the same concentration without decrease in overall signal intensity.

How it works

RePlay uses a nanoflow splitter and the unique RePlay valve to split the nanoLC eluent to a direct on-line analysis and to a Capture Cartridge.  During equilibration of the column, the RePlay valve switches to direct the captured chromatogram to the MS for a second analysis of the same injection.

RePlay analysis enables the following analytical possibilities:

  • Orthogonal MS Experiments - CID, ETD, HCD, IRMPD, ECD, MS3, precursor ion, etc.

  • Separate Mass Ranges - Separate the mass ranges into the direct and RePlay analyses to identify more ions within each mass range.

  • Improved Resolution - Slow down the chromatography during the RePlay analysis to improve chromatographic resolution with the use of a refocusing column.

  • Vary MS Conditions - Use a different collision energy during the RePlay analysis to improve MS/MS sensitivity.

  • Focused Analysis - Identify peaks of interest in the first analysis and use the RePlay analysis to dig deeper.

  • Exclusion and Inclusion List - Use exclusion and inclusion lists to look at additional ions or target those without a positive identification during the RePlay analysis.

  • Different Charge States - Look at doubly charged ions in the first analysis and triply charged and greater ions during the RePlay analysis to enhance ion fragmentation and improve structural information.

  • Extended Analysis - Slow down the chromatography without a refocusing column to broaden peak widths and provide more time during the RePlay analysis for data mining.

  • Positive and Negative Ion Analysis - Analyze in negative ion mode to identify PTMS, then switch to positive ion mode during the RePlay analysis to increase structural information.

  • Exploit Random Gains - Improve coverage from the random gains that generally occur during a repeat analysis of the same sample.
  • Reproducibility - Increase analytical completeness with a duplicate run for higher confidence.
Advion Announces RePlay for Proteomics
RePlay is a new nanoLC tool that provides proteomic researchers with two complementary MS analyses from one injection. (more)