LESA - Liquid Extraction Surface Analysis (Mode 3)

 

Liquid Extraction Surface Analysis (LESA) is the newest mode of operation for the TriVersa NanoMate, an automated chip-based nanoelectrospray source for mass spectrometry. Developed in collaboration with Oak Ridge National Laboratory, LESA enables simple, direct nanoESI mass spectrometric analysis from a variety of surfaces.                           

Benefits:

• Speed & Simplicity - no sample preparation
• More Information - long, stable ESI
• Sensitivity - lower detection limits than other surface analysis techniques

Analyze direct from any surface with extractable analytes such as:

• Thin tissue sections
• TLC plates and other planar separation media
• Dried blood spots (DBS) on paper
• Analyze MALDI plates for complementary information by ESI

The LESA capability is available as an upgrade to all previously installed TriVersa NanoMates as well as an option on new purchases, and is compatible with the majority of mass spectrometers.

On-Demand Web Presentation

Liquid Extraction Surface Analysis Mass Spectrometry (LESA-MS): A Novel Profiling Tool for Distribution and Metabolism Analysis                                                                                                                                                            Presented by: Daniel Eikel, PhD, Senior Application Scientist of Advion, Inc.

The Latest in Liquid Extraction Surface Analysis - LESA Clarity for AB SCIEX Mass Spec Systems                        Presented by: Thomas Covey, Principal Research Scientist of AB SCIEX

LESA:Liquid Extraction Surface Analysis: A New Surface Analysis Technique Using the Advion TriVersa NanoMate Presented by: Thomas Covey, Principal Research Scientist of AB SCIEX

Product Note

References

Direct detection of additives and degradation products from polymers by liquid extraction surface analysis employing            chip-based nanospray mass spectrometry                                                                                                                   Paine, M. R. L.; Barker, P. J.; Maclauglin, S. A.; Mitchell, T. W.; Blanksby, S. J.                                                             Rapid Commun Mass Spectrom. 2012 Feb 29;26(4):412-18. [Epub 2 012 Jan 15] 

Investigation of figopitant and its metabolites in rat tissue by combining whole body autoradiography with liquid extraction surface analysis mass spectrometry                                                                                                                     Schadt, S.; Kallback, S.; Almeida, R.; Sandel, J.                                                                                                            Drug Metab Dispos. [Epub 2011 Dec 19]. doi:10.1124/dmd.111.043562

Liquid extraction surface analysis mass spectrometry (LESA-MS) as a novel profiling tool for drug distribution and metabolism analysis: the terfenadine example                                                                                                          Eikel, D.; Vavrek, M.; Smith, S.; Bason, C.; Yeh, S.; Korfmacher, W. A.; Henion, J. D.                                                   Rapid Commun Mass Spectrom. 2011 Dec 15;25(23):3587-3596. [Epub 2011 Nov 16]

Direct sampling and analysis from solid-phase extraction cards using an automated liquid extraction surface analysis nanoelectrospray mass spectrometry system
Walworth, M.J.; Elnaggar, M.S.; Stankovich, J.J.; Witkowski, C.; Norris, J.L.; Van Berkel, G.J.
Rapid Commun Mass Spectrom. 2011 Sep 15;25(17):2389-96. doi: 10.1002/rcm.5132.

Liquid extraction surface analysis (LESA) of food surfaces employing chip-based nano-electrospray mass spectrometry
Eikel, D.; Henion, J.
Rapid Commun Mass Spectrom. 2011 Aug 30;25(16):2345-54. doi: 10.1002/rcm.5107.

Utility of spatially-resolved atmospheric pressure surface sampling and ionization techniques as alternatives to mass spectrometric imaging (MSI) in drug metabolism
Blatherwick, E.Q.; Van Berkel, G.J.; Pickup, K.; Johansson, M.K.; Beaudoin, M.E.; Cole, R.O.; Day, J.M.; Iverson, S.; Wilson, I.D.; Scrivens, J.H.; Weston, D.J.
Xenobiotica. 2011 Aug;41(8):720-34.

Comparative Lipidomics Profiling of Human Atherosclerotic Plaques
Stegemann, C.; Drozdov, I.; Shalhoub, J.; Humphries, J.; Ladroue, C.; Didangelos, A.; Baumert, M.; Allen, M.; Davies, AH.; Monaco, C.; Smith, A.; Xu, Q.; Mayr, M.
Circ Cardiovasc Genet. 2011 Apr 21. [Epub ahead of print]                                                                                       Article

Hemoglobin Variant Analysis via Direct Surface Sampling of Dried Blood Spots Coupled with High-Resolution Mass Spectrometry
Edwards, R.L.; Creese, A.J.; Baumert, M.; Griffiths, P.; Bunch, J.; Cooper, H.J.
Anal Chem. 2010 Mar 15;83(6):2265-70. [Epub 2011 Feb 22]
Article

Correlation of Skin Blanching and Percutaneous Absorption for Glucocorticoid Receptor Agonists by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging and Liquid Extraction Surface Analysis with Nanoelectrospray Ionization Mass Spectrometry
Marshall, P.; Toteu-Djomte, V.; Bareille, P.; Perry, H.; Brown, G.; Baumert, M.; Biggadike, K.
Anal. Chem. 2010 [Epub ahead of print]
Journal Abstract

* Fully automated liquid extraction-based surface sampling and ionization using a chip-based robotic nanoelectrospray platform
Kertesz, V.; Van Berkel, G.J.
J Mass Spectrom. 2010 Mar.; 45(3):252-60. [Epub 17 Dec 2009.]
Journal Abstract