Lipids Analysis

Reproducible and automated lipids analysis through long, stable spray times.

• Quantify and identify individual lipid species
• Consistent profile response from reproducible chip-based spray with up to 400 analyses per chip
• Faster analysis compared to conventional nanoelectrospray

Learn more about this application by viewing the following web presentation

Lipid Analysis with Andrej Shevchenko

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SELECT REFERENCES

COMPATIBLE MASS SPECTROMETERS

Following are peer reviewed articles, application notes, and posters demonstrating Advion's technology. You will need to sign in to access the documents listed below.
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Product Notes:

• Lipid Profiling Using the NanoMate
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Articles:

• "Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry"; Ejsing CS, Sampaio JL, Surendranath V, Duchoslav E, Ekroos K, Klemm RW, Simons K, Shevchenko A; Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2136-41. Epub 2009 Jan 27.

• "Accumulation of raft lipids in T-cell plasma membrane domains engaged in TCR signaling"; Zech T, Ejsing CS, Gaus K, de Wet B, Shevchenko A, Simons K, Harder T; EMBO J. 2009, 28:466:467.

• "Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics"; Matyash, Liebisch G, Kurzchalia TV, Shevchenko A, Schwudke D.; J. Lipid Res. 2008 , 49: 1137-1146

• "High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry"; Ståhlman M, Ejsing CS, Tarasov K, Perman J, Borén J, Ekroos K.; J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Feb 24. [Epub ahead of print]

• Top-Down Lipidomic Screens by Multivariate Analysis of High-Resolution Survey Mass Spectra"; Dominik Schwudke, et.al. - MPI of Molecular Cell Biology and Genetics; Analytical Chemistry; 5-3-2007
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• "Identification and Structure Determination of Sphingolipids from Sphingoid Bases to Sulfatides using QTrap and Chip-based NanoESI with Hybrid Q-TOF MS/MS"; M. Cameron Sullards - Georgia Institute of Technology; Keystone Symposia on Lipids; 2-24-2007
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• "Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning"; Ejsing CS, Duchoslav E, Sampaio J, Simons K, Bonner R, Thiele C, Ekroos K, Shevchenko A; Analytical Chemistry; 9-3-2006
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• "Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry"; C.S. Ejsing - Max Planck Institute of Molecular Cell Biology and Genetics; Journal of Mass Spectrometry; 2-3-2006
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• "Lipid-directed overexpression of mitochondrial glycerol-3-phosphate acyltransferase results in hepatic steatosis, increased triacylglycerol secretion and reduced fatty acid oxidation"; D. Linden - AstraZeneca R&D, Molndal; FASEB Journal; 2-3-2006
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• "Analysis of polyunsaturated aminophospholipid molecular species using"; R. Murphy et al - University of Colorado at Denver; Analytical Biochemistry; 2-1-2006
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• "Lipid Profiling by Multiple Precursor and Neutral Loss Scanning Driven by the Data-Dependent Acquisition"; A. Shevchenko - MPI of Molecular Cell Biology and Genetics; Analytical Chemistry; 1-3-2006
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• "Sphingolipidomics: High-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry"; Merrill Jr., A.H., Sullards, M.C., Allegood, J.C., Kelly, S., Wang, E.; Methods; 9-3-2005
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Application Notes:

• Essential Lipidomics Experiments using the LTQ Orbitrap Hybrid Mass Spectrometer; Authors: Thomas Moehring, Michaela Scigelova, Christer S. Ejsing, Dominik Schwudke, Andrej Shevchenko
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