Lipids Analysis

Faster lipids analysis with no sample-to-sample carryover with automated chip-based nanoelectrospray ionization on the TriVersa NanoMate.

• Quantify and identify individual lipid species with long,stable spray times
• Consistent profile response from reproducible chip-based spray with up to 400 analyses per chip
• Faster analysis compared to conventional nanoelectrospray
• No sample-to-sample carryover

How chip-based infusion for lipids analysis works »

Following are peer reviewed articles, application notes, and posters demonstrating Advion's technology. You will need to sign in to access the documents listed below. You must register before you sign in for the first time.

On-Demand Web Presentation

Automation of Multidimensional Mass Spectrometry-based Shotgun Lipidomics            Presented by - Xianlin Han, Ph.D., Professor, Diabetes and Obesity Research Center           Sanford-Burnham Medical Research Institute

Product Note

• Lipid Profiling Using the TriVersa NanoMate
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Articles:

• Comparative Lipidomics Profiling of Human Atherosclerotic Plaques
  Stegemann, C.; Drozdov, I.; Shalhoub, J.; Humphries, J.; Ladroue, C.; Didangelos, A.; Baumert, M.; Allen, M.; Davies, AH.; Monaco, C.; Smith, A.; Xu, Q.; Mayr, M.
  Circ Cardiovasc Genet. 2011 Apr 21. [Epub ahead of print]                                                               Article

• Increase of oxidant-related triglycerides and phosphatidylcholines in serum and small intestinal mucosa during development of intestinal polyp formation in Min mice
  Ikeda, K.; Mutoh, M.; Teraoka, N.; Nakanishi, H.; Wakabayashi, K.; Taguchi, R.
  Cancer Sci. 2011 Jan.;102(1):79-87 [Epub 2010 Oct 14]
  Article

• Orm family proteins mediate sphingolipid homeostasis Breslow, D.K.; Collins, S.R.; Bodenmiller, B.; Aebersold, R.; Simons, K.; Shevchenko, A.; Ejsing, C.S.; Weissman, J.S.
  Nature. 2010 Feb 25;463(7284):1048-53.
  Journal Abstract

• Determination of ganglioside composition and structure in human brain hemangioma by chip-based nanoelectrospray ionization tandem mass spectrometry
  Schiopu, C.; Flangea, C.; Capitan, F.; Serb, A.; Vukelić, Z.; Kalanj-Bognar, S.; Sisu, E.; Przybylski, M.; Zamfir, A.D.
  Anal Bioanal Chem. 2009 Dec;395(8):2465-77. [Epub 2009 Oct 16]
  Article

• A simple modification of a silicic acid lipid fractionation protocol to eliminate free fatty acids from glycolipid and phospholipid fractions
  Dickson, L.; Bull, I.D.; Gates, P.J.; Evershed, R.P.
  J Microbiol Methods. 2009 Sep;78(3):249-54. [Epub 2009 May 27]
  Journal Abstract

• Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network
  Klemm, R.W.; Ejsing, C.S.; Surma, M.A.; Kaiser, H.-J.; Gerl, M.J.; Sampaio, J.L.; de Robillard, Q.; Ferguson, C.; Proszynski, T.J.; Shevchenko, A.; Simons, K.
  J. Cell Biol . 2009 May 18;185(4):601-12. [Epub 2009 May 11]
  Journal Abstract

• High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry Ståhlman, M.; Ejsing, C.S.; Tarasov, K.; Perman, J.;Borén, J.; Ekroos K . J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Sep 15;877(26):2664-72. [Epub 2009 Feb 24]
  Journal Abstract

• Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry
  Ejsing, C.S.; Sampaio, J.L.; Surendranath, V.; Duchoslav, E.; Ekroos, K.; Klemm, R.W.; Simons, K.; Shevchenko, A.
  Proc Natl Acad Sci U.S.A. 2009 Feb 17;106(7):2136-41. [Epub 2009 Jan 27]
  Article

• Accumulation of raft lipids in T-cell plasma membrane domains engaged in TCR signaling
  Zech, T.; Ejsing, C.S.; Gaus, K.; de Wet, B.; Shevchenko, A.; Simons, K.; Harder, T.
  EMBO J. 2009, 28: 466–476.
  Article

• Microfluidics-based electrospray ionization enhances the intrasource separation of lipid classes and extends identification of individual molecular species through multi-dimensional mass spectrometry: development of an automated high-throughput platform for shotgun lipidomics
  Han, X.; Yang, K.; Gross, R.W. Rapid Commun Mass Spectrom. 2008 Jul;22(13):2115-24
  Journal Abstract

• Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics
  Matyash, V.; Liebisch, G.; Kurzchalia, T.V.; Shevchenko, A.; Schwudke D.;
  J. Lipid Res. 2008, 49: 1137-1146
  Article

• Shotgun Lipidomics Identifies a Paired Rule for the Presence of Isomeric Ether Phospholipid Molecular Species
  Yang, K.; Zhao, Z.; Gross, R. W.; Han, X.
  PLoS ONE. 2007 ; 2(12): e1368
  Article

• Detection of the abundance of diacylglycerol and triacylglycerol molecular species in cells using neutral loss mass spectrometry
  Murphy, R.C.; James, P.F.; McAnoy, A.M.; Krank, J.; Duchoslav, E.; Barkley, R.M.
  Anal. BioChem. 2007 , 366:59-70
  Article

• Osmolality, temperature and membrane lipid composition modulate the activity of betaine transporter BetP in Corynebacterium glutamicum
  Özcan, N.; Ejsing, C.S.; Shevchenko, A.; Lipski, A.; Morbach, S.; Krämer, R.
  J. Bacteriol. 2007 , 189(20): 7485-7496
  Article

• Electrospray mass spectrometry of human hair wax esters
  Fitzgerald, M.; Murphy, R.C.;
  J. Lipid Res. 2007 , 48:1231–1246
  Article

• FceRI and Thy-1 domains have unique protein and lipid compositions
  Surviladze, Z.; Harrison, K.A.; Murphy, R.C.; Wilson, B.S.;
  J. Lipid Res. 2007 , 48:1325–1335
  Article

• Top-Down Lipidomic Screens by Multivariate Analysis of High-Resolution Survey Mass Spectra

  Dominik Schwudke, et.al. - MPI of Molecular Cell Biology and Genetics; Analytical Chemistry; 5-3-2007
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• Identification and Structure Determination of Sphingolipids from Sphingoid Bases to Sulfatides using QTrap and Chip-based NanoESI with Hybrid Q-TOF MS/MS

  M. Cameron Sullards - Georgia Institute of Technology
  Keystone Symposia on Lipids; 2-24-2007                                                                Sign in to download.

• Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning; Ejsing CS, Duchoslav E, Sampaio J, Simons K, Bonner R, Thiele C, Ekroos K, Shevchenko A; Analytical Chemistry; 9-3-2006
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• Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry; C.S. Ejsing - Max Planck Institute of Molecular Cell Biology and Genetics; Journal of Mass Spectrometry; 2-3-2006
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• Liver-directed overexpression of mitochondrial glycerol-3-phosphate acyltransferase results in hepatic steatosis, increased triacylglycerol secretion and reduced fatty acid oxidation

  D. Linden - AstraZeneca R&D, Molndal; FASEB Journal; 2-3-2006
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• Analysis of polyunsaturated aminophospholipid molecular species using

  R. Murphy et al - University of Colorado at Denver; Analytical Biochemistry; 2-1-2006
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• Lipid Profiling by Multiple Precursor and Neutral Loss Scanning Driven by the Data-Dependent Acquisition

  A. Shevchenko - MPI of Molecular Cell Biology and Genetics                         Analytical Chemistry; 1-3-2006
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• Sphingolipidomics: High-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry

  Merrill Jr., A.H., Sullards, M.C., Allegood, J.C., Kelly, S., Wang, E.; Methods; 9-3-2005
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Application Notes:

• Essential Lipidomics Experiments using the LTQ Orbitrap Hybrid Mass Spectrometer; Authors: Thomas Moehring, Michaela Scigelova, Christer S. Ejsing, Dominik Schwudke, Andrej Shevchenko
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