Noncovalent Interactions

The TriVersa NanoMate is ideal for determining noncovalent interactions. Long, stable nanoelectrospray ionization enables rapid, inexpensive screening and determination of dissociation constants (Kd) for protein-ligand complexes.

• Perform reproducible noncovalent interactions protein-ligand screening up to 50 times faster and 100 times less expensive than NMR
• Nozzle-to-nozzle reproducibility enables the rapid determination of dissociation constants (Kd)

"The stability of the spray from the NanoMate allows us to generate high quality protein-ligand data within a couple of minutes. This data can usually be presented with no post application processing." -- Annick Parent, Sanofi-Aventis, Vitry-sur-Seine

“…we have demonstrated the power of nanoESI-MS…individual oligomeric species within complex mixtures of self-assembling molecules can be identified unambiguously… without the need for prior separation.” A.S. Ashcroft et al J Mol Biol 2006, 364(1):9-19

How chip-based nanoelectrospray ionization for noncovalent interactions works »

Following are peer reviewed articles, application notes, and posters demonstrating Advion's technology. You will need to sign in to access the documents listed below. You must register before you sign in for the first time.

Product Notes:

• Noncovalent Interactions Using Automated Chip-based Nanoelectrospray Ionization
  Sign in to download.

Articles:

• An integrative approach combining noncovalent mass spectrometry, enzyme kinetics and X-ray crystallography to decipher Tgt protein-protein and protein-RNA interaction
  Ritschel, T.; Atmanene, C.; Reuter, K.; Van Dorsselaer, A.; Sanglier-Cianferani, S.; Klebe, G.
  J Mol Biol. 2009 Nov 6;393(4):833-47. [Epub 2009 Jul 21]
  Journal Abstract

• HDX-ESI-MS Reveals Enhanced Conformational Dynamics of the Amyloidogenic Protein ß2-Microglobulin upon Release from the MHC-1
  Hodkinson, J.P.; Jahn, T.R.; Radford, S.E.; Ashcroft, A.E.
  J Am Soc Mass Spectrom. 2009 Feb.;20(2):278-86. [Epub 2008 Oct 17]
  Article

• Deciphering drift time measurements from travelling wave ion mobility spectrometry-mass spectrometry studies; David P. Smith, Tom W. Knapman, Iain Campuzano, Richard W. Malham, Joshua T. Berryman, Sheena E. Radford and Alison E. Ashcroft; Eur J Mass Spectrom (Chichester, Eng). 15, XXX–XXX (2009). doi: 10.1255/ejms.947. In Press.
  Sign in to access.
  
  
• Determination of active enzyme concentration using activity-based probes and direct mass spectrometric readout Ce´dric Bovet, Renato Zenobi Analytical Biochemistry 373 (2008) 380–382
  Sign in to request hardcopy.
  
  
• Identification of Endocrine-Disrupting Compounds Using Nanoelectrospray Ionization Mass Spectrometry Cédric Bovet, Marc Ruff, Arno Wortmann, Sylvia Eiler, Florence Granger, Bertran Gerrits, Dino Moras, and Renato Zenobi Chimia 62 (2008) 1–6
  Sign in to request hardcopy.
  
  
• Which Electrospray-Based Ionization Method Best Reflects Protein-Ligand Interactions Found in Solution? A Comparison of ESI, nanoESI, and ESSI for the Determination of Dissociation Constants with Mass Spectrometry Matthias Conradin Jecklin, David Touboul, Cédric Bovet, Arno Wortmann, and Renato Zenobi J Am Soc Mass Spectrom 2008, 19, 332–343
  Sign in to request hardcopy.
  
  
• Binding constant determination of high-affinity protein–ligand complexes by electrospray ionization mass spectrometry and ligand competition Arno Wortmann, Matthias C. Jecklin, David Touboul, Martin Badertscher and Renato Zenobi J. Mass Spectrom. (2007)
  Sign in to request hardcopy.
  
  
• Real-Time Monitoring of Protein Complexes Reveals their Quaternary Organization and Dynamics Alexander J. Painter, Nomalie Jaya, Eman Basha, Elizabeth Vierling, Carol V. Robinson, and Justin L.P. Benesch Chemistry & Biology 15, 246–253, March 2008
  Sign in to request hardcopy.
  
  
• Estrogen receptor–ligand complexes measured by chip-based nanoelectrospray mass spectrometry: An approach for the screening of endocrine disruptors                                         R. Zenobi - ETH Zurich; Protein Science; 3-3-2007
  Sign in to request hardcopy.
  
  
• Direct Observation of Oligomeric Species formed in the Early Stages of Amyloid Fibril Formation using Electrospray Ionisation Mass Spectrometry                                                          S. Radford - University of Leeds; Journal of Molecular Biology; 11-3-2006
  Sign in to request hardcopy.
• Monitoring the Zinc Affinity of the Metallo-ß-Lactamase CphA by Automated nanoESI-MS     Kris De Vriendt et al - University of Ghent; Journal of the American Society for Mass Spectrometry; 1-3-2006
  Sign in to request hardcopy.

• Studies on Ternary Metallo-ß Lactamase-Inhibitor Complexes Using Electrospray Ionization Mass Spectrometry
  Selevsek,N.; Tholey, A.; Heinzle, E.; Lienard, B.; Oldham, N.J.; Schofield, C.J.; Heinz, U.; Adolph, H.W.; Frere, J.M.;
  J. Am. Soc. Mass. Spectrom. 2006, 17:1000-1004
  Journal Abstract
  

• Real-time Monitoring of Enzymatic DNA Hydrolysis by Electrospray Ionization Mass Spectrometry                                                                                                                                            R.H.H. van den Heuvel, et al.; Nucleic Acids Research; 8-3-2005
  Sign in to request hardcopy.

• Evaluation of automated nano-electrospray mass spectrometry in the determination of non-covalent protein-ligand complexes
  De Vriendt, K.; Sandra, K., Desmet, T.; Nerinckx, W.; Van Beeumen, J.; Devreese, B.
  Rapid Commun. Mass Spectrom. 2004, 18:3061-3067
  Journal Abstract

• Use of a Microchip Device Coupled with Mass Spectrometry for Ligand Screening of a Multi-Protein Target                                                                                                                                 Catherine A. Keetch, Helena Hernandez, Alistair Sterling, Mark Baumert, Mark H. Allen, and Carol V. Robinson; Analytical Chemistry; 9-15-2003
  Sign in to request hardcopy.
  
  
• Quantitative Determination of Noncovalent Binding Interactions Using Automated Nanoelectrospray Mass Spectrometry                                                                                                 Sheng Zhang, Colleen K. Van Pelt, and David B. Wilson; Analytical Chemistry; 7-1-2003
  Sign in to request hardcopy.
  
  
• Automated Nano-Electrospray Mass Spectrometry for Protein-Ligand Screening by Noncovalent Interaction Applied to Human H-FABP and A-FABP                                                    K. Benkenstock, et.al. - Biovitrum AB; Journal of Biomolecular Screening; 6-3-2003
  Sign in to request hardcopy.

Application Notes:

• Studying RNA - Small Molecule Binding Using Automated Chip-based Nanoelectrospray Mass Spectrometry in Negative Ion Mode
  Sign in to download.
  
  
• Quantitative Determination of Noncovalent Protein-Ligand Interactions; Automated Chip-based Nanoelectrospray
  Sign in to download.

Scientific Posters:

• Automated Nanoelectrospray Mass Spectrometry From a Chip for Protein-Ligand Screening by Noncovalent Interaction Applied to Human H-FABP and A-FABP - K. Benkestock - Biovitrum AB and Royal Institute of Technology
  Sign in to download.
  
  
• Noncovalent Electrospray Ionization Mass Spectrometry: A Powerful Tool in Drug Discovery - K. Benkestock - Biovitrum
  Sign in to download.
  
  
• Quantitative Determination of Noncovalent Protein-Ligand Interactions Using Automated Nanoelectrospray Mass Spectrometry - S. Zhang
  Sign in to download.