Intact Protein Analysis

Conduct intact protein analysis including protein identification and protein characterization faster and more completely with automated chip-based nanoelectrospray.

• Identification of faint gel spots with high-quality electrospray ionization MS/MS
• Convert low-abundance MS/MS data into high-quality identifications with infusion for improved signal-to-noise
• Achieve 75 fmol detection limits of protein loaded on a gel
• Zero sample-to-sample carryover

“This workflow yielded up to 220% greater peptide coverage compared with gradient HPLC-ESI and provided protein identifications with up to a two-fold higher throughput rate than either HPLC-ESI approach…” P. Wright et al J Am Soc Mass Spectro 2007

“In the automated format of the TriVersa NanoMate, nano-electrospray measurements are robust, user-friendly and easily amenable for different protein samples while using very low amounts of spraying solution.” M. Mann et al Intl J Mass Spec 2007

How chip-based infusion for intact protein analysis works »

Following are peer reviewed articles, application notes, and posters demonstrating Advion's technology. You will need to sign in to access the documents listed below. You must register before you sign in for the first time.

Product Notes:

• Intact Protein Analysis Using Chip-Based Nanoelectrospray
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Articles:

• A protein epitope signature Tag (PrEST) library allows SILAC-based absolute quantification and multiplexed determination of protein copy numbers in cell lines                                      Zeiler, M.; Straube, W. L.; Uhlen, M.; Mann, M.                                                                                  Mol Cell Proteomics. [Epub 2011 Sep 30]

• Identification of the growth-hormone-releasing peptide-2 (GHRP-2) in a nutritional supplement
  Thomas, A.; Kohler, M.; Mester, J.; Geyer, H.; Schänzer, W.; Petrou, M.; Thevis, M.
  Drug Test Anal. 2010 Mar;2(3):144-8.
  

• Nanoelectrospray with ion-trap mass spectrometry for the determination of beta-casomorphins in derived milk products Juan-García, A.; Font, G.; Juan, C.; Picó, Y. Talanta. 2009 Nov 15;80(1):294-306. [Epub 2009 Jul 4]

• Two highly conserved cysteine residues in HPV16 L2 form an intramolecular disulfide bond and are critical for infectivity in human keratinocytes Campos, S.K.; Ozbun, M.A. PLoS One. 2009; 4(2):e4463. [Epub 2009 Feb 13]
  

• Towards Heat-stable Oxytocin Formulations: Analysis of Degradation Kinetics and Identification of Degradation Products Hawe, A.; Poole, R.; Romeijn, S.; Kasper, P.; van der Heijden, R.; Jiskoot, W. Pharm Res. 2009 Jul;26(7):1679-88. [Epub 2009 Apr 3]
  

• Detection and structural features of the betaB2-B3-crystallin heterodimer by radical probe mass spectrometry (RP-MS) Diemer, H.; Atmanene, C.; Sanglier, S.; Morrissey, B.; Van Dorsselaer, A.; Downard, K.M. J Mass Spectrom. 2009 May;44(5):803-12
  

• DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes Butala, M.; Busby, S.J.; Lee, D.J. Nucleic Acids Res. 2009 Apr;37(5):e37. [Epub 2009 Jan 30]
  

• Proteomics of the TRAP-induced platelet releasate Piersma, S.R.; Broxterman, H.J.; Kapci, M.; de Haas, R.R.; Hoekman, K.; Verheul, H.M.; Jiménez, C.R. J Proteomics. 2009 Feb 15;72(1):91-109. [Epub 2008 Nov 8]
  

• Computational analysis and experimental validation of gene predictions in Toxoplasma gondii
  Dybas, J. M., Madrid-Aliste, C. J., Che, F. Y., Nieves, E., Rykunov, D., Hogue, R., Weiss, L. M., Kim, K., Fiser, A.
  PLoS One. 2008 ;3(12):e3899. [Epub 2008 Dec 9]
  

• Technical Brief: a novel strategy for enrichment of trabecular meshwork protease proteome
  Picciani, R.; Junk, A. K.; Bhattacharya, S. K.
  Mol Vis. 2008 May 14;14:871-7
  

• An annotated catalogue of salivary gland transcripts in the adult female mosquito, Aedes aegypti
  Ribeiro, J. M. C.; Arcà, B.; Lombardo, F.; Calvo, E.; Phan, V. M.; Chandra, P. K.; Wikel, S. K.
  BMC Genomics. 2007 Jan 4;8:6
  

• Global analysis of community-associated methicillin-resistant Staphylococcus aureus exoproteins reveals molecules produced in vitro and during infection

  Christopher Burlak, Carl H. Hammer, Mary-Ann Robinson, Adeline R. Whitney, Martin J. McGavin, Barry N. Kreiswirth and Frank R. DeLeo                                            Cellular Microbiology (2007) 9(5), 1172-1190
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• Resolving Oligomers from Fully Grown Polymers with IMS-MS                                             Trimpin, et. al. Anal. Chem. 2007, 79, 7965 – 7974
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• A Systematic Evaluation of Chip-Based Nanoelectrospray Parameters for Rapid Identification of Proteins from a Complex Mixture                                                                                                       P. Wright - University of Sheffield                                                                                                        Journal of the American Society for Mass Spectrometry; 12-3-2007
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• Protein Identification Using Sequential Ion/Ion Reactions and Tandem Mass Spectrometry      D. Hunt - University of Virginia; PNAS; 7-3-2005
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• Investigation of the presence of b ions in electron capture dissociation mass spectra
  Cooper, H.J.;
  J. Am. Soc. Mass. Spectrom. 2005;16:1932–1940
  Journal Abstract

• Disposable Chromatography for a High-Throughput Nano-ESI/MS and Nano-ESI/MS-MS Platform
  Williams, J.G.; Tomer, K.B.
  J Am Soc Mass Spectrom. 2004 Sep;15(9):1333-40
  Article

• Chip-based nanoelectrospray mass spectrometry for protein characterization                          S. Zhang & C. K. Van Pelt; Expert Review in Proteomics - Copyright from Future Drugs LTD. All rights are reserved by Future Drugs Ltd.; 11-3-2004
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• Hybrid Ion Trap-Fourier Transform-Ion Cyclotron Resonance for High-Performance Analysis Lester Taylor; American Laboratory; 1-3-2004
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• Automated chip-based nanoelectrospray-mass spectrometry for rapid identification of proteins separated by two-dimensional gel electrophoresis
  Zhang, S.; Van Pelt, C.K.; Henion, J.D.
  Electrophoresis 2003;24:3620-3623
  Journal Abstract

• Surface-alkylated polystyrene monolithic columns for peptide analysis in capillary liquid chromatography-electrospray ionization mass spectrometry
  Huang, X.; Zhang, S.; Schultz, G.A.; Henion, J.
  Anal Chem. 2002 May 15;74(10):2336-44
  Journal Abstract
  

• Characterization of a fully automated nanoelectrospray system with mass spectrometric detection for proteomic analyses                                                                                                           C. Van Pelt, S. Zhang, J. Henion; Journal of Biomolecular Techniques; 6-3-2002
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• Nanoelectrospray peptide mapping revisited: Composite survey spectra allow high dynamic range protein characterization without LCMS on an orbitrap mass spectrometer                      M. Mann - Max-Planck-Institute for Biochemistry                                                                          International Journal of Mass Spectrometry; 1-3-1997
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Application Notes:

• Fully Automated High Throughput Accurate Mass Determination Using FT-ICR Mass Spectrometry
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• Identification of HPLC-Fractionated; Ultimate “Peak Parking” by Fractionation
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• Identification of HPLC-Fractionated Tryptic Peptides Using the NanoMateTM 100 System; Ultimate "Peak Parking" by Fractionation
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• Identify Faint Colloidal Coomassie Blue Gel Spots in 6 Minutes with Electrospray
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• Protein Identification Using the NanoMate™ 100 System Following 2-D Gel Separation; Automated Nanoelectrospray for Proteomics
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Scientific Posters:

• A New Workflow for Iterative Bottom-up Analysis of Gas Phase Enriched Human Plasma Proteins Using In-line Fraction Collection and nanoESI FTMS - J. Sutton - Thermo BRIMS Center
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• Coupling a Nanoflow Liquid Chromatography Column to Automated chip-Based nanospray Emitters for Tandem Mass Spectrometric Protein Identification - X. Huang - Advion
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• Coupling NanoLC Columns to the ESI Chip for Enhanced Sensitivity, Ionization Efficiency, and Reduced Ion Supression - G. Schultz
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• Direct Elution of Preconcentrated Protein Digests from Packed Pipette Tips to Chip-based NanoESI/MS/MS for Enhanced Protein Identification Sensitivity - X. Huang
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• Rapid, Automated Nanoelectrospray MS/MS Analysis of Excised 2-D Gel Spots - S. Zhang - Advion BioSciences
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• Rearrangement of Disulfide Bridges in UV-Exposed Goat Lactalbumin: Evidence by NanoESI/MS and MALDI-TOF-FOT Mass Spectrometry - A. Sterling
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• The use of hydrogen-deuterium exchange to investigate the role of structural confromation and protein folding of the amyloidogenic protein B2-microglobulin - J.P. Hodkinson - University of Leeds
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