Protein Identification

Protein identification in six minutes per sample with greater peptide coverage. 

• Identification of faint gel spots with high-quality electrospray MS/MS
• Convert low-abundance MS/MS data into high-quality identifications with infusion for improved signal-to-noise
• Achieve 75 fmol detection limits of protein loaded on a gel
• Zero sample-to-sample carryover

Learn more about this application by viewing the following web presentation

Advancing Proteomics with Ion/Ion Chemistry - ETD and Beyond

“This workflow yielded up to 220% greater peptide coverage compared with gradient HPLC-ESI and provided protein identifications with up to a two-fold higher throughput rate than either HPLC-ESI approach…” “P. Wright et al J Am Soc Mass Spectro 2007, xx, xxx”

“In the automated format of the TriVersa NanoMate, nano-electrospray measurements are robust, user-friendly and easily amenable for different protein samples while using very low amounts of spraying solution.” “M. Mann et al Intl J Mass Spec 2007, xx, xxx”

How it works >>

Following are peer reviewed articles, application notes, and posters demonstrating Advion's technology. You will need to sign in to access the documents listed below.
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Product Notes:

• Protein Identification Using the Nanomate
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Articles:

• "Global analysis of community-associated methicillin-resistant Staphylococcus aureus exoproteins reveals molecules produced in vitro and during infection".
  Christopher Burlak, Carl H. Hammer, Mary-Ann Robinson,  Adeline R. Whitney,  Martin J. McGavin,  Barry N. Kreiswirth  and Frank R. DeLeo(2007)
  Cellular Microbiology (2007) 9(5), 1172-1190
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• "Resolving Oligomers from Fully Grown Polymers with IMS-MS ". Trimpin, et. al. Anal. Chem. 2007, 79, 7965 – 7974
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• "IN PRESS: A Systematic Evaluation of Chip-Based Nanoelectrospray Parameters for Rapid Identification of Proteins from a Complex Mixture"; P. Wright - University of Sheffield; Journal of the American Society for Mass Spectrometry; 12-3-2007
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• "Protein Identification Using Sequential Ion/Ion Reactions and Tandem Mass Spectrometry"; D. Hunt - University of Virginia; PNAS; 7-3-2005
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• "Chip-based nanoelectrospray mass spectrometry for protein characterization"; S. Zhang & C. K. Van Pelt; Expert Review in Proteomics - Copyright from Future Drugs LTD. All rights are reserved by Future Drugs Ltd.; 11-3-2004
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• "Hybrid Ion Trap-Fourier Transform-Ion Cyclotron Resonance for High-Performance Analysis"; Lester Taylor; American Laboratory; 1-3-2004
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• "Characterization of a fully automated nanoelectrospray system with mass spectrometric detection for proteomic analyses"; C. Van Pelt, S. Zhang, J. Henion; Journal of Biomolecular Techniques; 6-3-2002
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• "IN PRESS: Nanoelectrospray peptide mapping revisited: Composite survey spectra allow high dynamic range protein characterization without LCMS on an orbitrap mass spectrometer"; M. Mann - Max-Planck-Institute for Biochemistry; International Journal of Mass Spectrometry; 1-3-1997
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Application Notes:

• Fully Automated High Throughput Accurate Mass Determination Using FT-ICR Mass Spectrometry
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• Identification of HPLC-Fractionated; Ultimate “Peak Parking” by Fractionation
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• Identification of HPLC-Fractionated Tryptic Peptides Using the NanoMateTM 100 System; Ultimate "Peak Parking" by Fractionation
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• Identify Faint Colloidal Coomassie Blue Gel Spots in 6 Minutes with Electrospray
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• Protein Identification Using the NanoMate™ 100 System Following 2-D Gel Separation; Automated Nanoelectrospray for Proteomics
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Scientific Posters:

• A New Workflow for Iterative Bottom-up Analysis of Gas Phase Enriched Human Plasma Proteins Using In-line Fraction Collection and nanoESI FTMS - J. Sutton - Thermo BRIMS Center
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• Coupling a Nanoflow Liquid Chromatography Column to Automated chip-Based nanospray Emitters for Tandem Mass Spectrometric Protein Identification - X. Huang - Advion
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• Coupling NanoLC Columns to the ESI Chip for Enhanced Sensitivity, Ionization Efficiency, and Reduced Ion Supression - G. Schultz
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• Direct Elution of Preconcentrated Protein Digests from Packed Pipette Tips to Chip-based NanoESI/MS/MS for Enhanced Protein Identification Sensitivity - X. Huang
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• Rapid, Automated Nanoelectrospray MS/MS Analysis of Excised 2-D Gel Spots - S. Zhang - Advion BioSciences
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• Rearrangement of Disulfide Bridges in UV-Exposed Goat Lactalbumin: Evidence by NanoESI/MS and MALDI-TOF-FOT Mass Spectrometry - A. Sterling
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• The use of hydrogen-deuterium exchange to investigate the role of structural confromation and protein folding of the amyloidogenic protein B2-microglobulin - J.P. Hodkinson - University of Leeds
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