Metabolite Identification

Metabolite Identification with the TriVersa NanoMate offers greater than 10 times more sensitivity than LC/MS/MS for detection of low level, potentially active or toxic recirculating metabolites.

• Characterize “hot” radio-labeled LC fractions
• Obtain structural information on trace-level metabolites by infusing collected HPLC fractions
• Low flow rates enable signal averaging for improved signal-to-noise
• Rapidly switch between mass spectrometry platforms to obtain positive ion, precursor ion, neutral loss scanning or MSn analysis as well as accurate mass
• Less analyte required to get the same data quality versus LC/MS/MS

How fraction collection and infusion works for metabolite identification »

Following are peer reviewed articles, application notes, and posters demonstrating Advion's technology. You will need to sign in to access the documents listed below. You must register before you sign in for the first time.

Product Notes

• Metabolite Identification Using Automated Chip-based Nanoelectrospray Ionization
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Articles:

• Advances in structure elucidation of small molecules using mass spectrometry
  Kind, T.; Fiehn, O.
  Bioanal. Rev. 2010; 2(1):23-60
  Article

• (13)C Isotope-Labeled Metabolomes Allowing for Improved Compound Annotation and Relative Quantification in Liquid Chromatography-Mass Spectrometry-based Metabolomic Research
  Giavalisco, P.; Kohl, K.; Hummel, J.; Seiwert, B.; Willmitzer, L.
  Anal Chem. 2009 Jul 9. [Epub ahead of print]
  Article

• Discovering natural products from myxobacteria with emphasis on rare producer strains in combination with improved analytical methods
  Garcia, R.O.; Krug, D.; Müller, R. Methods Enzymol. 2009;458:59-91
  Journal Abstract

• Negative ion 'chip-based' nanospray tandem mass spectrometry for the analysis of flavonoids in glandular trichomes of Lychnophora ericoides Mart. (Asteraceae)
  Gobbo-Neto. L.; Gates, P.J.; Lopes, N.P.
  Rapid Commun Mass Spectrom. 2008 Dec;22(23):3802-8
  Journal Abstract

• High-resolution direct infusion-based mass spectrometry in combination with whole 13C metabolome isotope labeling allows unambiguous assignment of chemical sum formulas
  Giavalisco, P.; Hummel, J.; Lisec, J.; Inostroza, A.C.; Catchpole, G.; Willmitzer, L. Anal Chem. 2008 Dec 15;80(24):9417-25
  Journal Abstract

• Rapid metabolite identification with sub parts-permillion mass accuracy from biological matrices by direct infusion nanoelectrospray ionization after clean-up on a ZipTip and LTQ/Orbitrap mass spectrometry; Erve, J..; DeMaio, W.; Talaat, R. Rapid Commun Mass Spectrom. 2008; 22: 3015–3026
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• Dual negative precursor ion scan approach for rapid detection of glutathione conjugates using liquid chromatography/tandem mass spectrometry
  Mahajan, M.K.; Evans, C.A. Rapid Commun Mass Spectrom. 2008 Apr;22(7):1032-40
  Article

• Measurement of Total Acid Number (TAN) and TAN Boiling Point Distribution in Petroleum Products by Electrospray Ionization Mass Spectrometry. K. Qian, et. al.                                    Anal Chem. 2008 Jan 8
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• Comparison of a two-dimensional liquid chromatography/mass spectrometry approach with a chip-based nanoelectrospray device for structural elucidation of metabolites in a human ADME study using a quadrupole time-of-flight mass spectrometer; Trunzer, M.; Graf, D.; Kiffe, M. Rapid Commun Mass Spectrom. 2007;21(6):937-44
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• Dynamic Range and Mass Accuracy of Wide-Scan Direct Infusion Nanoelectrospray FourierTransform Ion Cyclotron Resonance Mass Spectrometry-Based Metabolomics Increased by the Spectral Stitching Method
  Southam, A.D.; Payne, T.G.; Cooper, H.J.; Arvanitis, T.N.; Viant, M.R.
  Anal. Chem. 2007; 79:4595-4602
  Journal Abstract

• Quantitation of Cotinine in Nonsmoker Saliva Using Chip-Based Nanoelectrospray Tandem Mass Spectrometry; Tompkins, B.A.; Van Berkel G.J.; Jenkins, R.A.; Counts R.W. J Anal Toxicol. 2006 Apr;30(3):178-86(9)
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• The Combination of Liquid Chromatography/Tandem Mass Spectrometry and Chip-Based Infusion for Improved Screening and Characterization of Drug Metabolites                              Roland F. Staack, Emmanuel Varesio, and Gerard Hopfgartner                                                  Rapid Commun Mass Spectrom.; 1-3-2005
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• Controlled Protein Precipitation in Combination with Chip-Based Nanospray Infusion Mass Spectrometry. An Approach for Metabolomics Profiling of Plasma
  Boernsen, K.O.; Gatzek, S.; Imbert, G.;
  Anal. Chem. 2005; 77(22):7255-64
  Journal Abstract

• Identification of a novel glutathione adduct of diclofenac, 4'-hydroxy-2'-glutathion-deschloro-diclofenac, upon incubation with human liver microsomes
  Yu, L. J.; Chen, Y.; Deninno, M. P.; O’Connell, T. N.; Hop, C. E.
  Drug Metab Dispos. 2005 Apr.;33(4):484-8. [Epub 2005 Jan 7]
  Article

• Metabolite fingerprinting: detecting biological features by independent component analysis
  Scholz, M.; Gatzek, S.; Sterling, A.; Fiehn, O.; Selbig, J.;
  Bioinformatics. 2004, 20(15):2447-54 [Epub 2004 Apr 15]
  Article

• Quantitation of midazolam in human plasma by automated chip-based infusion nanoelectrospray tandem mass spectrometry
  Kapron, J.T.; Pace, E.; Van Pelt, C.K.; Henion, J.
  Rapid Commun Mass Spectrom. 2003;17(18):2019-26
  Journal Abstract

Scientific Posters:

• Accelerating Metabolite Identification Using Automated Chip-Based Infusion Nanoelectrospray on a Q-TOF - C. Van Pelt
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• Use of Automated Nanoelectrospray for Drug Metabolite Structure Elucidation - D. Borts - GlaxoSmithKline
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