PTM Analysis

PTM Analysis with automated chip-based nanoelectrospray ionization

Fully characterize targeted Post-Translational Modifications (PTMs) missed by LC/MS/MS with the TriVersa NanoMate.

  • Analyze targeted PTMs in complex mixtures using online nanoLC for increased sensitivity, reproducibility and ionization efficiency
  • Obtain chip-based nanoelectrospray quality MS/MS sequence information from multiply charged ions
  • Characterize targeted Post-Translational Modifications with extended analysis time of each fraction while allowing for collision energy optimization, data dependent averaging, neutral loss, precursor ion, and MSn experiments

"The NanoMate has provided us with excellent stability, and sensitivity in negative ion mode, on both our ESI-QToF and FTICR instruments. This has enabled us to not only to identify novel biological carbohydrate species, but to characterise them and their noncovalent interactions by MS and tandem MS within a single experiment. This is of crucial importance in our glycoanalyses for biomedical and clinical purposes where we are required to obtain as much compositional and structural information as possible from very low sample amounts." -- Alina Zamfir and Jasna Peter-Katalinic, University of Muenster

How LC/MS fraction collection for PTM analysis works »

Following are peer reviewed articles, application notes, and posters demonstrating Advion's technology. You will need to sign in to access the documents listed below. You must register before you sign in for the first time.

Product Notes:

Articles:

  • Analysis of human hippocampus gangliosides by fully-automated chip-based nanoelectrospray tandem mass spectrometry
    Vukelica, Z.; Zareib, M.; Peter-Katalinic, J.; Zamfir, A.D.
    J. Chromatogr. A. 2006;1130:238-245
    Journal Abstract

  • Determination of Mammillaria gracillis N-glycan patters by ESI Q-TOF mass spectrometry
    Balen, B.; Zamfir, A.D.; Vakhrushev, S.; Krsnik-Rasol, M.; Peter Katalinic, J.
    Croat. Chem. Acta. 2006;78:463-477
    Journal Abstract

  • Characterization of Protein Glycosylation Using Chip-based Infusion Nanoelectrospray Linear Ion Trap Tandem Mass Spectrometry                                                                                     S. Zhang and D. Chelius - Thermo Electron; Journal of Biomolecular Techniques; 6-3-2004
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  • Coupling of fully automated chip electrospray to Fourier transform ion cyclotron resonance mass spectrometry for high-performance glycoscreening and sequencing                                 J. Peter-Katalinic, et.al. - U. Muenster                                                                                               Rapid Communications in Mass Spectrometry; 12-3-2004
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  • Fully Automated Chip-Based Mass Spectrometry for Complex Carbohydrate System Analysis A. Zamfir, et.al. - University of Munster, Germany; Analytical Chemistry; 4-3-2004
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  • Fully-Automated Chip-Based Nanoelectrospray Tandem Mass Spectrometry of Gangliosides from Human Cerebellum                                                                                                                        Z. Vukelic, et.al. - University of Muenstery; American Society for Mass Spectrometry; 9-3-2004
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  • Off-line capillary electrophoresis/fully automated nanoelectrospray chip quadrupole time-of-flight mass spectrometry and tandem mass spectrometry for glycoconjugate analysis           A. Zamfir, et.al. - University of Muenster; Journal of Mass Spectrometry; 10-3-2004
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  • Ultralow-Volume Fraction Collection from NanoLC Columns for Mass Spectrometric Analysis of Protein Phosphorylation and Glycosylation                                                                                     T. Corso et al. - Advion; Analytical Chemistry - ASAP Article; 1-3-2006
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Application Notes:

  • Data-Dependent™ Neutral-Loss Mass Spectrometry
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  • Identification of Biantennary N-Linked Glycan Structure; Using chip-based nanoelectrospray MSn to provide stable, extended infusion time
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  • Identification of Phosphorylation Sites in alpha-Casein; Using Chip-Based Nanoelectrospray with Automated Data-Dependent Neutral Loss Mass Spectrometry
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  • Protein Glycosylation Using Chip-based Nanoelectrospray MSn Mass Spectrometry; Stable, extended infusion time for glycosylation mapping
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Scientific Posters

  • Identification of Phosphorylation Sites Using Automated Chip-Based Nanoelectrospray Coupled to a Linear Ion Trap Mass Spectrometer - T. Corso & S. Zhang
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  • Mapping Phosphorylation Sites of Yeast Myosin Using Chip-Based Nanoelectrospray Quadrupole TOF and 3D Ion Trap Mass Spectrometry - S. Zhang
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  • NanoLC Fraction analysis by Chip-Based nanoelectrospray for Improved Glycopeptide Characterization - T. Corso et al. - Advion
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Lipidome Profiling of Colon Adenocarcinoma Cell lines