QA/QC Antibodies/Glycans

Cut the amount of time required to identify and characterize monoclonal antibodies for projects involving QA proteins using the TriVersa NanoMate.

• Rapid amino acid sequence information for intact proteins within minutes
• Find post-translational modification sites not detected during an LC/MS run
• Identify and confirm low-level monoclonal antibody isoforms through the combination of top-down and bottom-up approaches

How LC/MS fraction collection for QA/QC antibodies works »

Following are peer reviewed articles, application notes, and posters demonstrating Advion's technology. You will need to sign in to access the documents listed below. You must register before you sign in for the first time.

Articles:

• The N-glycome of human plasma
  Stumpo, K.A.; Reinhold, V.N.
  J Proteome Res. 2010 Sep 3;9(9):4823-30.
  Journal Abstract

• A multi-method approach toward de novo glycan characteriztion: A Man-5 case-study
  Prien, J.M.; Prater, B.D.; Cockrill, S.L.
  Glycobiology. 2010 May;20(5):629-47. [Epub 2010 Jan 27]
  Journal Abstract

• Glutamine-linked and non-consensus asparagine-linked oligosaccharides present in human recombinant antibodies define novel protein glycosylation motifs
  Valliere-Douglass, J.F.; Eakin, C.M.; Wallace, A..; Ketchem, R.R.; Wang, W.; Treuheit, M.J.; Balland, A.
  J Biol Chem. 2010 May 21;285(21):16012-22. [Epub 2010 Mar 16]
  Journal Abstract

• Mass spectrometric-based stable isotopic 2-aminobenzoic acid glycan mapping for rapid screening of biotherapeutics
  Prien, J.M.; Prater, B.D.; Qin, Q.; Cockrill, S.L.
  Anal Chem. 2010 Feb 15;82(4):1498-508
  Journal Abstract

• Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry
  Flangea, C.; Schiopu, C.; Sisu, E.; Serb, A.; Przybylski, M.; Seidler, D.G.; Zamfir, A.D.
  Anal Bioanal Chem. 2009 Dec;395(8):2489-98. [Epub 2009 Oct 14]
  Journal Abstract

• Asparagine-linked oligosaccharides present on a non-consensus amino acid sequence in the CH1 domain of human antibodies
  Valliere-Douglass, J.F.; Kodama, P.; Mujacic, M.; Brady, L.J.; Wang, W.; Wallace, A.; Yan, B.; Reddy, P.; Treuheit, M.J.; Balland, A.
  J Biol Chem. 2009 Nov 20;284(47):32493-506. [Epub 2009 Sep 18]
  Journal Abstract

• Extending Mass Spectrometry Contribution to Therapeutic Monoclonal Antibody Lead Optimization: Characterization of Immune Complexes Using Noncovalent ESI-MS
  Atmanene, C.; Wagner-Rousset, E.; Malissard, M.; Chol, B.; Robert, A.; Corvaïa, N.; Dorsselaer, A.V.; Beck, A.
  Anal Chem. 2009 Aug 1;81(15):6364-73
  Journal Abstract

• Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis
  Almeida, R.; Mosoarca, C.; Chirita, M.; Udrescu ,V.; Dinca, N.; Vukelic, Z.; Allen, M.; Zamfir, A.D.
  Anal Biochem. 2008 Jul 1;378(1):43-52. [Epub 2008 Mar 28]
  Journal Abstract

Application Notes:

• Rapid Characterization of Monoclonal Antibodies by Top-Down Sequencing
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• Site Specific Characterization of Monoclonal Antibodies
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