Top-Down Proteomics

The best mass accuracy, resolution, and sensitivity from FTMS instruments on projects involving Top-Down Proteomics sequencing using stable, reproducible chip-based nanoelectrospray ionization.

• Characterize phosphorylation, glycosylation, and other post-translational modification sites
• Low sample volume (2 -10 microliters) and the ability to return unused samples for maximum flexibility
• Automated infusion for acquisition of multiple scan MS/MS data

"We are encouraged by the results we have obtained using TriVersa NanoMate and LTQ Orbitrap demonstrating their suitability for top-down proteomics of small proteins. Sub-picomole amounts of intact proteins proved sufficient to obtain high quality MS, MS2 and MS3 spectra, with mass accuracies in the few ppm range."

Top-down protein sequencing and MS3 on a hybrid linear quadrupole ion trap orbitrap mass spectrometer

B. Macek, L. Waanders, J.V. Olsen, M. Mann  Mol.Cell.Prot. 2006; 5: 949-958

How LC/MS fraction collection for top-down proteomics works »

Following are peer reviewed articles, application notes, and posters demonstrating Advion's technology. You will need to sign in to access the documents listed below. You must register before you sign in for the first time.

Product Notes:

• FTMS/ECD Analysis with the TriVersa NanoMate
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Articles:

• Pressurized pepsin digestion in proteomics: An automatable alternative to trypsin for integrated top-down bottom-up proteomics
  López-Ferrer, D.; Petritis, K.; Robinson, E. W.; Hixson, K. K.; Tian, Z.; Lee, J. H.; Lee, S. W.; Tolic, N.; Weitz, K. K.; Belov, M. E.; Smith, R. D.; Pasa-Tolic, L.
  Mol Cell Proteomics. 2011 Feb;10(2):M110.001479. [Epub 2010 Jul 12]
  Journal Article

• Top-Down Proteomics Reveals Novel Protein Forms Expressed in Methanosarcina acetivorans Ferguson, J.T.; Wenger, C.D.; Metcalf, W.W.; Kelleher, N.L.
  J Am Soc Mass Spectrom. 2009 Sep;20(9):1743-50. [Epub 2009 Jun 6]
  Journal Abstract

• Top-down high-resolution mass spectrometry of cardiac myosin binding protein C revealed that truncation alters protein phosphorylation state
  Ge, Y.; Rybakova, I.N.; Xu, Q.; Moss, R.L.
  Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12658-63. [Epub 2009 Jun 16]
  Article

• Delineating Anopheles gambiae coactivator associated arginine methyltransferase 1 automethylation using top-down high resolution tandem mass spectrometry
  Kuhn, P.; Xu, Q.; Cline, E.; Zhang, D.; Ge, Y.; Xu, W.
  Protein Sci. 2009 Jun;18(6):1272-80
  Journal Abstract

• Activated Ion Electron Capture Dissociation (AI ECD) of proteins: synchronization of infrared and electron irradiation with ion magnetron motion
  Mikhailov, V.A.; Cooper, H.J.
  J Am Soc Mass Spectrom. 2009 May; 20(5):763-71. [Epub 2008 Dec 31]
  Article

• An integrated top-down and bottom-up strategy for broadly characterizing protein isoforms and modifications
  Wu, S.; Lourette, N.M.; Tolic, N.; Zhao, R.; Robinson, E.W.; Tolmachev, A.V.; Smith, R.D.; Pasa-Tolic, L.
  J Proteome Res. 2009 Mar;8(3):1347-57
  Journal Abstract

• At-line coupling of UPLC to chip-electrospray-FTICR-MS                                                                 Li, X.; Fekete, A.; Englmann, M.; Frommberger, M.; Lv, S.; Chen, G.; Schmitt-Kopplin, P.       Anal Bioanal Chem. 2007 Sep 5
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• Top-down protein sequencing and MS3 on a hybrid linear quadrupole ion trap-orbitrap mass spectrometer"; M. Mann - Max Planck Institute for Biochemistry; Molecular & Cellular Proteomics; 2-3-2006
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• Top-Down Approaches for Measuring Expression Ratios of Intact Yeast Proteins Using Fourier Transform Mass Spectrometry                                                                                                N. Kelleher et al - University of Illinois Urbana; Analytical Chemistry; 1-3-2006
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• Investigation of the Presence of b Ions in Electron Capture Dissociation Mass Spectra             H. Cooper - University of Birmingham; JASMS; 12-3-2005
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• Construction of a Hybrid Quadrupole/Fourier-Transform Spectrmeter for Versatile MS/MS Above 10 kDa                                                                                                                                         S.M. Patrie, et.al. - University of Illinois at Urbana-Champaign                                                        J. Am. Soc. Mass Spectrom.; 11-3-2004
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• Improved Molecular Weight-Based Processing of Intact Proteins for Analysis by Q-FTMS with Automated MS/MS                                                                                                                                       Du, Y, et.al. - University of Illinois at Urbana-Champaign; Analytical Chemistry; 5-3-2004
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• Molecular-Level Description of Proteins from Saccharomyces cerevisiae using Q-FT Mass Spectrometry for Top Down Proteomics                                                                                              F. Meng, et.al. - University of Illinois at Urbana-Champaign; Analytical Chemistry; 4-3-2004
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• Strategies for Automating Top Down Proteomics with Q-FT ICR MS                                            S.M. Patrie, et.al. - University of Illinois at Urbana-Champaign                                                         Int. J. Mass Spectrom.; 4-3-2004
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• Targeted Analysis and Discovery of Posttranslational Modifications in Proteins from Methonogenic Archaea by Top-Down MS                                                                                            N. Kelleher, et.al. - Univeristy of Illinois; PNAS; 3-2-2004
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Application Notes:

• Identification of 2D Gel Fractionated Intact Proteins Using FT-ICR Mass Spectrometry; High Throughput
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Scientific Posters:

• Split SIEVE: Informatic Support for Differential Split LC-FTMS Experiments - M. Askenazi - Thermo BRIMS Center
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• Top-Down Analysis of Low Molecular Weight Human Plasma Proteome Using a Hybrid Ion Trap-Fourier Transform Mass Spectrometer - ThermoElectron Corporation
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