Mode 3: Liquid Extraction Surface Analysis (LESA®)
Liquid Extraction Surface Analysis (LESA) is the newest mode of operation for the TriVersa NanoMate, an automated chip-based nanoelectrospray source for mass spectrometry. Developed in collaboration with Oak Ridge National Laboratory, LESA enables simple, direct nanoESI mass spectrometric analysis from a variety of surfaces.
- Speed & Simplicity – no sample preparation
- More Information – long, stable ESI
- Sensitivity – lower detection limits than other surface analysis techniques
Analyze direct from any surface with extractable analytes such as:
- Thin tissue sections
- TLC plates and other planar separation media
- Dried blood spots (DBS) on paper
- Analyze MALDI plates for complementary information by ESI
The LESA capability is available as an upgrade to all previously installed TriVersa NanoMates as well as an option on new purchases, and is compatible with the majority of mass spectrometers.
- Direct detection of additives and degradation products from polymers by liquid extraction surface analysis employing chip-based nanospray mass spectrometry Paine, M. R. L.; Barker, P. J.; Maclauglin, S. A.; Mitchell, T. W.; Blanksby, S. J. Rapid Commun Mass Spectrom. 2012 Feb 29;26(4):412-18. [Epub 2012 Jan 15]
- Utilizing the inherent electrolysis in a chip-based nanoelectrospray emitter system to facilitate selective ionization and mass spectrometric analysis of metallo alkylporphyrins Van Berkel, G.J.; Vertesz, V. Anal Bioanal Chem [Epub 2012 Jan 26] DOI 10.1007/s00216-011-5676-x
- Investigation of figopitant and its metabolites in rat tissue by combining whole body autoradiography with liquid extraction surface analysis mass spectrometry Schadt, S.; Kallback, S.; Almeida, R.; Sandel, J. Drug Metab Dispos. [Epub 2011 Dec 19]. doi:10.1124/dmd.111.043562
- Liquid extraction surface analysis mass spectrometry (LESA-MS) as a novel profiling tool for drug distribution and metabolism analysis: the terfenadine example Eikel, D.; Vavrek, M.; Smith, S.; Bason, C.; Yeh, S.; Korfmacher, W. A.; Henion, J. D. Rapid Commun Mass Spectrom. 2011 Dec 15;25(23):3587-3596. [Epub 2011 Nov 16]
- Direct sampling and analysis from solid-phase extraction cards using an automated liquid extraction surface analysis nanoelectrospray mass spectrometry system Walworth, M.J.; Elnaggar, M.S.; Stankovich, J.J.; Witkowski, C.; Norris, J.L.; Van Berkel, G.J. Rapid Commun Mass Spectrom. 2011 Sep 15;25(17):2389-96. doi: 10.1002/rcm.5132.
- Liquid extraction surface analysis (LESA) of food surfaces employing chip-based nano-electrospray mass spectrometry Eikel, D.; Henion, J. Rapid Commun Mass Spectrom. 2011 Aug 30;25(16):2345-54. doi: 10.1002/rcm.5107.
- Utility of spatially-resolved atmospheric pressure surface sampling and ionization techniques as alternatives to mass spectrometric imaging (MSI) in drug metabolism Blatherwick, E.Q.; Van Berkel, G.J.; Pickup, K.; Johansson, M.K.; Beaudoin, M.E.; Cole, R.O.; Day, J.M.; Iverson, S.; Wilson, I.D.; Scrivens, J.H.; Weston, D.J. Xenobiotica. 2011 Aug;41(8):720-34.
- Comparative Lipidomics Profiling of Human Atherosclerotic Plaques Stegemann, C.; Drozdov, I.; Shalhoub, J.; Humphries, J.; Ladroue, C.; Didangelos, A.; Baumert, M.; Allen, M.; Davies, AH.; Monaco, C.; Smith, A.; Xu, Q.; Mayr, M. Circ Cardiovasc Genet. 2011 Apr 21. [Epub ahead of print] Article
- Hemoglobin Variant Analysis via Direct Surface Sampling of Dried Blood Spots Coupled with High-Resolution Mass Spectrometry Edwards, R.L.; Creese, A.J.; Baumert, M.; Griffiths, P.; Bunch, J.; Cooper, H.J. Anal Chem. 2010 Mar 15;83(6):2265-70. [Epub 2011 Feb 22] Article
- Correlation of Skin Blanching and Percutaneous Absorption for Glucocorticoid Receptor Agonists by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging and Liquid Extraction Surface Analysis with Nanoelectrospray Ionization Mass Spectrometry Marshall, P.; Toteu-Djomte, V.; Bareille, P.; Perry, H.; Brown, G.; Baumert, M.; Biggadike, K. Anal. Chem. 2010 [Epub ahead of print] Journal Abstract
- * Fully automated liquid extraction-based surface sampling and ionization using a chip-based robotic nanoelectrospray platform Kertesz, V.; Van Berkel, G.J. J Mass Spectrom. 2010 Mar.; 45(3):252-60. [Epub 17 Dec 2009.] Journal Abstract
