Application Notes

This Webinar was originally presented March 7, 2024 on LabRoots.com ABSTRACT The latest purification systems are not like those of the past — you do not have to choose between flash and PrepLC. Now, you can have both with the ability to decide your needs with one user-friendly system. In this webinar, you will learn …

Jack Henion, Changtong Hao, Daniel Eikel, Olof Beck, and Peter Stambeck* Abstract Increased acceptance of cannabis containing the psychoactive component, Δ9-tetrahydrocannabinol (THC), raises concerns about the potential for impaired drivers and increased highway accidents. In contrast to the “breathalyzer” test, which is generally accepted for determining the alcohol level in a driver, there is no …

This Webinar was originally presented November 9, 2023 on LabRoots.com ABSTRACT In this webinar, you’ll learn how to address your lab’s purification pain-points with methods and instrumentation to maximize your budget, time and increase overall efficiency. During this session you will discover: How to select the ideal purification solution for your lab’s specific needs How …

This whitepaper explores the use of alternative chromatography technology for faster, more efficient analysis over traditional LC/MS runs. Plate Express™ is a device that provides a simple, automated means of obtaining mass spectra directly from TLC plates, combined with the Advion Interchim Scientific® expression® CMS (Compact Mass Spectrometer) creating a technique known as TLC/CMS. Using …

Cameron J. Stouffer and R. Kenneth Marcus * Abstract Cloth swipes are a fairly common sampling modality, having relevance across a wide variety of applications including environmental analysis, forensics, and bioassays. One of the biggest attractions of swipes (and related paper substrates) is the ease of sample collection and transportation, where just the swipe of …

Oligonucleotides have gained significant attention in biopharmaceutical development due to their ability to modulate gene or protein expression. This application note aims to demonstrate the HPLC/UV analysis of multiple oligo samples and utilize HPLC/CMS analysis to determine their molecular weight.

In this application note, we use whey protein isolate and two casein macro peptides as an example to demonstrate the isolation and purification of bioactive peptide components with a system comprised of a puriFlash® 5.250 prepLC coupled online to an expression® CMS detector.

Shaun N. Robertson, Fadi Soukarieh, Thomas M. White, Miguel Camara, Manuel Romero*, and Rian L. Griffiths* Abstract Previously, metabolites diffused or secreted from microbial samples have been analyzed via liquid chromatography–mass spectrometry (LC–MS) approaches following lengthy extraction protocols. Here, we present a model system for growing biofilms on discs before utilizing rapid and direct surface …

A book of experiments for your students The expression® CMS (Compact Mass Spectrometer) is the ideal instrument for the teaching lab thanks to its small size, ease of use, and one-click software. This pocket guide of classroom experiments offers students the ability to gain hands-on instrument experience by eliminating complex and difficult sample preparation.

In this application note, we demonstrate a complete solution for the synthesis, purification, and quantitation of Gd(III)-DOTA, an ionic imaging contrast agent with better chemical stability and lower toxicity compared to the currently used Gd-(DTPA).

In this application note, we present a fast method for routine analysis of small volume blood samples for key toxic and essential elements using the Advion Interchim Scientific® SOLATION® Inductively Coupled Plasma Mass Spectrometer (ICP-MS) using a simple “dilute and shoot” sample preparation.

Iohexol is a widely used non-ionic imaging agent that improves contrast for x–ray analysis. Its low osmolality allows for a rapid clearance via the kidney, preventing reabsorption and further metabolization. This makes iohexol a compound with a better safety profile compared to other imaging agents. In this application note, a simple and accurate HPLC-CMS method …

Recorded February 2, 2023: LIVE Location, the 2023 European Winter Conference on Plasma Spectrochemistry Speakers: Janez Ilaš, Ph. D., Professor, University of Ljubljana Fadi Abou-Shakra, Director of Advion Interchim Scientific® ICP-MS Portfolio Join Advion Interchim Scientific® with guest speaker from the University of Ljubljana, Faculty of Pharmacy, to learn more about a new novel sample …

Maximize your analytical spectroscopy workflow in pharmaceutical applications—even with limited space at the bench. This session features innovative solutions for streamlining everyday needs in the lab, including reaction monitoring utilizing fast, prep-free sample introduction techniques for answers in seconds. You will also learn the latest technology in heavy metal analysis, as well as purification tools …

In this application note we present a method for routine analysis of 21 elements using the Advion Interchim Scientific® SOLATION® Inductively Coupled Plasma Mass Spectrometer (ICP-MS). A group of unknown soil samples and a CRM were digested using EPA 3051a and analyzed according to method 6020a requirements.

In this application note, we show how Cyanidin-3-glucoside can be extracted from black rice and purified with a prepLC/Flash system. The amount and purity of Cyanidin-3-glucoside was measured utilizing a certified reference standard.

In this application note, a method to separate and purify green tea catechins with preparative liquid chromatography (Prep-LC) using the puriFlash® 5.250 system is demonstrated. An additional HPLC-UV-MS method is also demonstrated for compound confirmation and purity analysis.

In this application note, a method to separate and purify 3 curcuminoids from turmeric powder using thin layer chromatography (TLC) with mass spectrometry and flash chromatography is demonstrated.

In this application note, we describe methods of isolating natural products from green tea and turmeric powder by mass-directed fraction collection during flash chromatography and preparative LC. For demonstration purposes, the isolated compounds were then additionally confirmed by Atmospheric Solids Analysis Probe (ASAP®) MS or HPLC-MS.