Application Note

Microorganism Surface Analysis Using the TriVersa NanoMate for Liquid Extraction Surface Analysis of Bacteria and Fungi


Liquid Extraction Surface Analysis Mass Spectrometry (LESA-MS) has been utilized for determination of small molecules, lipids and proteins from a variety of surfaces such as tissue sections, plant material, medical devices, TLC plates or DBS cards. It allows direct surface analysis without sample preparation from spot sizes of 1 mm diameter by aspiration of extraction solvent into a pipette tip, formation of a liquid junction at the intended target location and extraction of the analytes of interest. In the final step, analytes are ionized by static nano electrospray and detected in the mass spectrometer.