Executive Summary:
This poster was presented at MSACL 2018 US in Palm Springs, CA.
Learn more about Liquid Extraction Surface Analysis PLUS LC separation with the Advion TriVersa NanoMate® featuring new LESAPlus technology.
ABSTRACT: Large-scale metabolic profiling requires the development of novel economical high-throughput analytical methods to facilitate characterization of systemic metabolic variation in population phenotypes. We report a fit-forpurpose direct infusion nanoelectrospray high-resolution mass spectrometry (DI-nESI-HRMS) method with time-of-flight detection for rapid targeted parallel analysis of over 40 urinary metabolites. The newly developed 2 min infusion method requires <10 μL of urine sample and generates high-resolution MS profiles in both positive and negative polarities, enabling further data mining and relative quantification of hundreds of metabolites. Here we present optimization of the DI-nESI-HRMS method in a detailed step-by-step guide and provide a workflow with rigorous quality assessment for large-scale studies.
From the Journal of Proteome Research, Published February 2017
The TriVersa NanoMate® LESA® is the latest in chip-based electrospray ionization technology from Advion Interchim Scientific®. It combines the benefits of liquid chromatography, mass spectrometry, chip-based infusion, fraction collection and direct surface analysis into one integrated ion source platform. It allows scientists to obtain more information from complex samples than LC/MS alone.
Quant Express, an addition to Advion’s user-friendly Mass Express 4.0 software suite, features quantitation to create a fully integrated software system for expression CMS users.
Published by the Royal Society of Chemistry
Liquid Extraction Surface Analysis (LESA) is a new, high throughput tool for ambient mass spectrometry. A solvent droplet is deposited from a pipette tip onto a surface and maintains contact with both the surface and the pipette tip for a few seconds before being re-aspirated. The technique is particularly suited to the analysis of trace materials on surfaces due to its high sensitivity and low volume of sample removal. In this work, we assess the suitability of LESA for obtaining detailed chemical profiles of fingerprints, oral fluid and urine, which may be used in future for rapid medical diagnostics or metabolomics studies. We further show how LESA can be used to detect illicit drugs and their metabolites in urine, oral fluid and fingerprints. This makes LESA a potentially useful tool in the growing field of fingerprint chemical analysis, which is relevant not only to forensics but also to medical diagnostics. Finally, we show how LESA can be used to detect the explosive material RDX in contaminated artificial fingermarks.
ChipSoftX is an entirely new operating software for the TriVersa NanoMate automated nanoelectrospray source. Besides improvement in program compatibility with Windows and integration of existing software features, it also provides access to the new Developers Kit – a platform for customized method development with direct access to robot controls allowing entirely novel analysis workflows such as LESAPLUS.
Greater than 10 times more sensitive than LC/MS/MS for detection of low level, potentially active or toxic recirculating metabolites.
Presented by: John P. Shockcor, Director of Life Sciences Business Development, Waters Corp., Visiting Fellow, Dept. of Biochemistry, University of Cambridge, UK
Description: Profiling low level components in a complex mixture of small molecules can be a challenging task. Although it may be possible to detect many low level components in a complex mixture, characterization is often hindered because fragmentation of these low level components yields peaks below the limit of detection. This problem can be alleviated by using a TriVersa NanoMate assisted approach. In this webinar we will describe how a TriVersa NanoMate coupled to a SYNAPT G2 Hybrid QTof Ion-Mobility Mass Spectrometer can provide critical fragmentation information needed to characterize low level components in lipidomics, drug metabolism studies and natural product profiling. This approach is ideally suited to the use of time-aligned-parallel fragmentation (TAP) which are illustrated by a number of examples.
Presented by: Dr. Daniel Eikel, Sr. Application Scientist – Advion, Inc.
Description: Liquid extraction surface analysis mass spectrometry (LESA-MS) is a novel mass spectrometry-based surface-profiling technique (Kertez, et al., 2009) that can be utilized in drug distribution and metabolism studies. Potential advantages of LESA-MS are as follows:
In this webinar, Dr. Daniel Eikel expands upon his publication (Henion, et al., 2011), discussing how LESA-MS can provide complementary information to the gold standards in this field: quantitative whole body autoradiography (QWBA) and whole organ LC-MS/MS, or other MS based approaches such as MALDI-MSI. He demonstrates how they have evaluated LESA-MS by studying the drug distribution and metabolism of terfenadine in mouse, and through direct comparison, proved that LESA-MS appears to be more informative than a comparable MALDI-MSI experiment and reflects the literature-known metabolism and distribution pattern for terfendine and its metabolite fexofenadine.