In this application note, discover how the SPE-LV6 PFAS-Free system streamlines solid phase extraction with full automation for sample volumes from 100 μL to 5 L. This innovative workstation eliminates PFAS contamination, ensuring reliable, repeatable results across diverse matrices. Learn how it accelerates method development, boosts productivity, and delivers clean, consistent performance with zero cross-contamination.
Determination of Intact Lactoferrin Content in Dairy Products Using U-HPLC-UV
Lactoferrin is a multifunctional dairy protein (antimicrobial, antiviral, antioxidant,…) used in many nutritional applications. Part of its properties is based on its ability to bind Iron. This binding ability has an impact on its analytical characterization, which has to be considered for accurate determination.
The AOAC Int. issued a call for methods for determination of Bovine Lactoferrin (bLF) in Infant and Adult/Pediatric Nutritional Formula based on Standard Method Performance Requirements (SMPR) 2020.005 [Table 1]. Official AOAC Method is based on LC-MS-MS with tryptic digestion which is quite time consuming, and non-specific of soluble bLF.
This poster describes a method for the quantification of intact and native Lactoferrin in dairy products using U-HPLC-UV after Cation Exchange Extraction, meeting SMPR requirements. We also discuss the importance of Iron saturation and its impacts on Extinction Coefficient, linked to the analytical standard used.