Effect-directed analysis of bioactive compounds in Cannabis sativa L. by high-performance thin-layer chromatography


Justus Liebig University Giessen, University of Modena and Reggio Emilia


Effect-directed analysis (EDA) by the combination of high-performance thin-layer chromatography (HPTLC) with biologi- cal and enzymatic assays represents one of the latest tools available for the rapid bioprofiling of complex matrices, such as plant extracts. In this ambit, the aim of this project was the non-targeted screening of inflorescence extracts from ten different hemp varieties for components exhibiting radical scavenging, antibacterial, enzyme inhibiting and estrogen-like effects.

The characterization of two prominently multipotent bioactive com- pound zones was finally achieved by HPTLC-HRMS and preliminary assigned as cannabidiolic acid and cannabidivarinic acid.

HTPLC analysis was coupled via the Advion Plate Express® TLC Plate Reader.