Carbohydrates are non-chromophoric compounds which can often lead to flat signals when using UV detection only. In this application note, we show carbohydrate purification with ELSD and how such detection can help in purification.
The ELSD detector that AIS proposes is capable of providing several benefits to the user, including:
- No need to define gain depending on the loading.
- The ability to see more compounds (especially temperature sensitive or volatile) thanks to low temperature technology.
- Removal of water response – In this application we used water and 35°C was enough to remove water response and get a flat signal.
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