In this application note, we show how Cyanidin-3-glucoside can be extracted from black rice and purified with a prepLC/Flash system. The amount and purity of Cyanidin-3-glucoside was measured utilizing a certified reference standard.
Extraction, Identification and Purification of (-)- Epigallocatechin Gallate From Green Tea
In this application note, a method to separate and purify green tea catechins with preparative liquid chromatography (Prep-LC) using the puriFlash® 5.250 system is demonstrated. An additional HPLC-UV-MS method is also demonstrated for compound confirmation and purity analysis.
Extraction and Purification of 3 Curcuminoids from Turmeric Powder
In this application note, a method to separate and purify 3 curcuminoids from turmeric powder using thin layer chromatography (TLC) with mass spectrometry and flash chromatography is demonstrated.
Analytical Sciences Synthesis & Purification: Consumables, Reagents & Instruments
The new edition of our “Analytical Sciences, Synthesis & Purification” catalog is available now! Offering a full suite of innovative products and technologies from Advion Interchim Scientific, this catalog features over 20,000 products ranging from consumables and reagents to instrumentation.
Not only a product resource, the catalog features in-depth scientific information to improve your daily workflow, including sample preparation tips and tricks, analysis technologies such as GC and LC/MS, and help selecting the best consumables for your workflow or purification.
Take advantage of this extensive guide to boost your lab with:
- 20,000 Products
- 12 Chapters
- Technicals Tips
- New Systems
Cannabinoid Analysis in Edible Samples Using the Advion Interchim Scientific AVANTTM UHPLC/PDA System
In this application note, a simple, cost-effective UHPLC/UV method is introduced for analysis of cannabinoids in edible samples (examples shown are gummy bears, chocolates and cookies) using the Advion Interchim Scientific AVANT™ UHPLC/PDA System.
Mass Directed Fraction Collection of Natural Products: Examples from Turmeric & Green Tea Extract
Flash chromatography has traditionally used UV absorption as the main method of detection for compounds during a purification process. While UV absorption is broadly applicable to many classes of compounds, it has limited specificity to individual compounds in a mixture and misses classes of compounds that do not carry chromophores.
Mass-directed fraction collection gives users the ability to collect fractions based on mass spectrometry detection (MS) which is based on ions specific to individual compounds and provides specific molecular information. This allows for simplification in the overall purification process and greater confidence in the identity of each isolated compound. Here we describe methods of isolating natural products from green tea and turmeric powder by mass-directed fraction collection during flash chromatography and preparative LC.
This poster was presented at the 2021 ASMS national conference in Philadelphia, PA.
The Combined Application of the Caco-2 Cell Bioassay Coupled with In Vivo (Gallus gallus) Feeding Trial Represents an Effective Approach to Predicting Fe Bioavailability in Humans
USDA, University of Connecticut
Abstract
Research methods that predict Fe bioavailability for humans can be extremely useful in evaluating food fortification strategies, developing Fe-biofortified enhanced staple food crops and assessing the Fe bioavailability of meal plans that include such crops. In this review, research from four recent poultry (Gallus gallus) feeding trials coupled with in vitro analyses of Fe-biofortified crops will be compared to the parallel human efficacy studies which used the same varieties and harvests of the Fe-biofortified crops. Similar to the human studies, these trials were aimed to assess the potential effects of regular consumption of these enhanced staple crops on maintenance or improvement of iron status. The results demonstrate a strong agreement between the in vitro/in vivo screening approach and the parallel human studies.
LC/MS analysis was complete using the Advion expression Compact Mass Spectrometer (CMS). Instrumentation and data acquisition were controlled by Advion Mass Express software
Nucleotides, micro- and macro-nutrients, limonoids, flavonoids, and hydroxycinnamates composition in the phloem sap of sweet orange
University of Florida, USDA, Kansas State University
Abstract
Currently, the global citrus production is declining due to the spread of Huanglongbing (HLB). HLB, otherwise known as citrus greening, is caused by Candidatus Liberibacter asiaticus (CLas) and is transmitted by the Asian citrus psyllids (ACP), Diaphorina citri Kuwayama.
In this study, we investigated the micro- and macro-nutrients, nucleotides, and others secondary metabolites of phloem sap from pineapple sweet orange. The micro- and macro-nutrients were analyzed using inductively coupled plasma-mass spectroscopy (ICP-MS) and inductively coupled plasma-optical emission spectroscopy (ICP-OES). Nucleotides and other secondary metabolites analysis was accomplished by reversed phase HPLC coupled with UV, fluorescence detection, or negative mode electrospray ionization mass spectrometry (ESI-MS) using the Advion expression Compact Mass Spectrometer (CMS).
Alterations in the Intestinal Morphology, Gut Microbiota, and Trace Mineral Status Following Intra-Amniotic Administration (Gallus gallus) of Teff (Eragrostis tef) Seed Extracts
Cornell University, Kaiser Permanente Fontana, Bar-Ilan University
Abstract
The consumption of teff (Eragrostis tef), a gluten-free cereal grain, has increased due to its dense nutrient composition including complex carbohydrates, unsaturated fatty acids, trace minerals (especially Fe), and phytochemicals. This study utilized the clinically-validated Gallus gallus intra amniotic feeding model to assess the effects of intra-amniotic administration of teff extracts versus controls using seven groups: (1) non-injected; (2) 18Ω H2O injected; (3) 5% inulin; (4) teff extract 1%; (5) teff extract 2.5%; (6) teff extract 5%; and (7) teff extract 7.5%. The treatment groups were compared to each other and to controls. Our data demonstrated a significant improvement in hepatic iron (Fe) and zinc (Zn) concentration and LA:DGLA ratio without concomitant serum concentration changes, up-regulation of various Fe and Zn brush border membrane proteins, and beneficial morphological changes to duodenal villi and goblet cells. No significant taxonomic alterations were observed using 16S rRNA sequencing of the cecal microbiota. Several important bacterial metabolic pathways were differentially enriched in the teff group, likely due to teff’s high relative fiber concentration, demonstrating an important bacterial-host interaction that contributed to improvements in the physiological status of Fe and Zn. Therefore, teff appeared to represent a promising staple food crop and should be further evaluated.
Analysis was performed by LC/MS using the Advion Interchim Scientific expression® Compact Mass Spectrometer (CMS).
Yacon (Smallanthus sonchifolius) flour soluble extract improve intestinal bacterial populations, brush border membrane functionality and morphology in vivo (Gallus gallus)
Federal University of Viçosa, Cornell University
This study evaluates the effects of intra-amniotic administration of yacon (Smallanthus sonchifolius) flour soluble extracts (YFSE) on intestinal bacterial populations, brush border membrane (BBM) functionality and morphology, by using the Gallus gallus model.
Highlights:
- Yacon flour soluble extract (YFSE) increased the relative abundance of bifidobacterial and Lactobacillus.
- YFSE upregulated the expression of iron and zinc transporters.
- YFSE decreased gene expression of inflammation marker and hepcidin.
- YFSE increased glycogen concentration in pectoral muscle.
- YFSE improved BBM functionality and intestinal morphology.
Analysis was performed by LC/MS using the Advion expression Compact Mass Spectrometer (CMS).