Blood Analysis using the Advion Interchim Scientific® SOLATION® ICP-MS

In this application note, we present a fast method for routine analysis of small volume blood samples for key toxic and essential elements using the Advion Interchim Scientific® SOLATION® Inductively Coupled Plasma Mass Spectrometer (ICP-MS) using a simple “dilute and shoot” sample preparation.

Analysis of Iohexol using the Advion Interchim Scientific® AVANT® HPLC and expression® CMS System

Iohexol is a widely used non-ionic imaging agent that improves contrast for x–ray analysis. Its low osmolality allows for a rapid clearance via the kidney, preventing reabsorption and further metabolization. This makes iohexol a compound with a better safety profile compared to other imaging agents.

In this application note, a simple and accurate HPLC-CMS method for iohexol analysis is introduced.

Streamlined Benchtop Mass Spectrometry for the Pharmaceutical Industry

Maximize your analytical spectroscopy workflow in pharmaceutical applications—even with limited space at the bench. This session features innovative solutions for streamlining everyday needs in the lab, including reaction monitoring utilizing fast, prep-free sample introduction techniques for answers in seconds. You will also learn the latest technology in heavy metal analysis, as well as purification tools to maximize your scale-up efforts.

As a viewer, you will learn more about:

  • How to obtain direct mass analysis from TLC plates in seconds
  • Prep-free mass sampling techniques such as a direct analysis probe
  • How heavy metal analysis technology has improved for maximum use in the pharmaceutical lab
  • How scale-up needs can be met with the latest purification technologies

Analytical Sciences Synthesis & Purification: Consumables, Reagents & Instruments

The new edition of our “Analytical Sciences, Synthesis & Purification” catalog is available now! Offering a full suite of innovative products and technologies from Advion Interchim Scientific, this catalog features over 20,000 products ranging from consumables and reagents to instrumentation.

Not only a product resource, the catalog features in-depth scientific information to improve your daily workflow, including sample preparation tips and tricks, analysis technologies such as GC and LC/MS, and help selecting the best consumables for your workflow or purification.

Take advantage of this extensive guide to boost your lab with:

  • 20,000 Products
  • 12 Chapters
  • Technicals Tips
  • New Systems

Direct Screening of Cocaine on Money Note Surfaces by OPSI/CMS Analysis

Cocaine is a globally used illicit drug and its widespread use remains a significant public health concern. It is known that US paper currency in general circulation is contaminated with cocaine. There are several possible explanations for this: contamination due to handling during drug deals, the use of rolled up bills for snorting or inhalation, and cross contamination between bills in circulation. The analytical methods for cocaine analysis on paper currency are normally HPLC/UV, GC/MS or LC-MS/MS, however, all of these methods require significant sample preparation before analysis.

In this poster, a quick and direct screening method for detection of cocaine on paper currency is introduced using the Touch Express Open Port Sampling Interface (OPSI) on the Advion expression® CMS.

This poster was presented at ASMS 2021 in Philadelphia, PA, USA.

Natural Products Purification – Improved Workflow with Mass Spec

Lab Manager Tech Trends Webinar. Recorded August 11, 2021.

This webinar features the Interchim PuriFlash® flash purification and preparative HPLC system and demonstrates separation of natural products, purification of specific target molecules and the combination of mass spectrometry detection of the same both for product confirmation and mass directed fraction collection using an Advion expression® compact mass spectrometer.

As a viewer, you will learn more about:

  • Natural product separation and analyte isolation from tea and herbs/spices starting from TLC separation
  • Column selection criteria for flash chromatography and preparative reverse phase HPLC
  • Integrating mass spectrometry detection of analytes from TLC plates and collected fractions
  • Mass spectrometry directed fraction collection to simplify the entire workflow and automate purification processes

A Green One-Pot Multicomponent Synthesis of 4H-Pyrans and Polysubstituted Aniline Derivatives of Biological, Pharmacological, and Optical Applications Using Silica Nanoparticles as Reusable Catalyst

Guru Ghasidas University, University of South Dakota

A one-pot practical, efficient, and environmentally benign multicomponent synthesis of 4H-pyrans and polysubstituted aniline derivatives of biological, pharmacological, and optical applications has been developed using a very mild, neutral, and reusable silica nanoparticles as catalyst. The 4H-pyran derivatives were synthesized by a three component reaction of an aldehyde, malononitrile, and 5,5-dimethyl-1,3-cyclohexanedione or ethyl acetoacetate at room temperature or refluxing in ethanol. Alternatively, polysubstituted anilines were synthesized via a four component reaction of an aldehyde, a ketone, and two equivalents of malononitrile in ethanol.

Mass spec analysis was carried out using an Advion expression Compact Mass Spectrometer (CMS).

Mass Directed Fraction Collection for Preparative SFC Collection of a 0.2% Impurity

This is one from a set of two application notes. In this application note, we determine the purity of collection of a small (0.2%) impurity in a concentrated sample mix by mass-directed fraction collection using supercritical fluid chromatography (SFC) coupled with the Advion expression Compact Mass Spectrometer (CMS).

For the other application note on the recovery and purification of two, three component standard mixtures, click here.

Advion co-authored with a major pharmaceutical partner to create two application notes highlighting the benefits of supercritical fluid chromatography (SFC) coupled to a compact mass spectrometer for mass directed fraction collection. These application notes conclude that mass‐directed fraction collection with a simplified passive splitter on the Preparative JASCO SFC coupled with expression CMS system increases purification selectivity by mass directed fraction collection while maintaining excellent recovery and purity. The MS accepted high concentrations of sample material with peak shapes trailing edges almost indistinguishable from the UV trace. Low, Middle and High isocratic flow rates all showed comparable MS source clearance leading to minimal tailing beyond the UV. Gradients further minimized peak tailing as expected on the UV and this was also seen on the TIC and XIC. Peak purities for all main band purifications were ≥99.9% pure and minor impurity collections showed equivalent success yielding 100% pure fractions. Acceptable recoveries for all collected fractions were 94% or better.

Anti-Trypanosomal Evaluation of Ximenia Americana Root Bark and Chromatographic-Mass Spectrometric Profile

Nigerian Institute for Trypanosomiasis, University of Jos, University of Yaoundé


Medicinal plants are the richest bio-resource of drugs for traditional systems of medicine, modern medicines, food supplements, pharmaceutical intermediates and chemical entities for synthetic drugs. Human African Trypanosomiasis (HAT) is a challenging and deadly disease due to its complex epidemiology and clinical presentations.  This study was conducted to investigate anti-trypanosomal action of Ximenia americana root bark on Trypanosoma brucei brucei using various solvent extracts and to develop thin layer (TLC-MS) and liquid chromatography-mass spectrometric (LC-MS) profiles of the plant.  Soxhlet extraction was used to obtain acetone, 70% ethanol total extracts in addition to n-hexane, dichloromethane, ethyl acetate and methanol fractions by sequential extraction. The inhibitory activity of the various extracts was compared by testing against T. b. brucei using isometamidium chloride as standard drug. The most active extract was separated by solid-phase extraction (C18 stationary phase) to obtain fractions which were profiled by TLC-MS (+ESI) and LC-MS. It was observed that anti-trypanosomal activity of acetone (16.83% yield) and 70% ethanol (18.23% yield) were comparable. However, methanol extract exhibited the highest activity with 99.18%, 97.5% and 87.50% inhibition at 3 h incubation (room temperature) using 1000 µg, 500 µg and 250 µg concentrations respectively. The activities at 1000 µg for methanol extract and isometamidium chloride were comparable with 95% CI [-1.10, 1.77]. TLC-MS and LC-MS analyses suggested gallic acid, 2,3,4,5-tetrahydroxybenzoic acid, 2ʹ,5-dimethoxyflavone, quercetin, dihydroquercetin and sesquiterpene when compared with literature database. This study presents data that could be useful in standardisation and preparation of alternative medicine in the treatment of African trypanosomiasis.

Analysis was performed by TLC/MS using the Advion expression Compact Mass Spectrometer (CMS) and Plate Express TLC Plate Reader.