In this application note, we compare the use of a flash chromatography system with a UV detector alone versus the same system paired with a mass spectrometer. The data highlights the impact on workflow efficiency and underscores the importance of selecting the appropriate splitter.
The specificity of MS detection improves the efficiency of the purification workflow (compared to traditional Flash and HPLC).
In this application note, we present a fast method for routine analysis of small volume blood samples for key toxic and essential elements using the Advion Interchim Scientific® SOLATION® Inductively Coupled Plasma Mass Spectrometer (ICP-MS) using a simple “dilute and shoot” sample preparation.
Iohexol is a widely used non-ionic imaging agent that improves contrast for x–ray analysis. Its low osmolality allows for a rapid clearance via the kidney, preventing reabsorption and further metabolization. This makes iohexol a compound with a better safety profile compared to other imaging agents.
In this application note, a simple and accurate HPLC-CMS method for iohexol analysis is introduced.
Maximize your analytical spectroscopy workflow in pharmaceutical applications—even with limited space at the bench. This session features innovative solutions for streamlining everyday needs in the lab, including reaction monitoring utilizing fast, prep-free sample introduction techniques for answers in seconds. You will also learn the latest technology in heavy metal analysis, as well as purification tools to maximize your scale-up efforts.
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The new edition of our “Analytical Sciences, Synthesis & Purification” catalog is available now! Offering a full suite of innovative products and technologies from Advion Interchim Scientific, this catalog features over 20,000 products ranging from consumables and reagents to instrumentation.
Not only a product resource, the catalog features in-depth scientific information to improve your daily workflow, including sample preparation tips and tricks, analysis technologies such as GC and LC/MS, and help selecting the best consumables for your workflow or purification.
Take advantage of this extensive guide to boost your lab with:
In this application note, we used the Advion Interchim Scientific puriFlash® 5.250 to purify five active ingredients found in over-the-counter (OTC) and prescription drugs including caffeine, glafenine, ketoprofen, flavone, and fenofibrate, by a preparative purification workflow. The compounds were then confirmed using the expression® compact mass spectrometer.
Cocaine is a globally used illicit drug and its widespread use remains a significant public health concern. It is known that US paper currency in general circulation is contaminated with cocaine. There are several possible explanations for this: contamination due to handling during drug deals, the use of rolled up bills for snorting or inhalation, and cross contamination between bills in circulation. The analytical methods for cocaine analysis on paper currency are normally HPLC/UV, GC/MS or LC-MS/MS, however, all of these methods require significant sample preparation before analysis.
In this poster, a quick and direct screening method for detection of cocaine on paper currency is introduced using the Touch Express Open Port Sampling Interface (OPSI) on the Advion expression® CMS.
This poster was presented at ASMS 2021 in Philadelphia, PA, USA.
Lab Manager Tech Trends Webinar. Recorded August 11, 2021.
This webinar features the Interchim PuriFlash® flash purification and preparative HPLC system and demonstrates separation of natural products, purification of specific target molecules and the combination of mass spectrometry detection of the same both for product confirmation and mass directed fraction collection using an Advion expression® compact mass spectrometer.
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Guru Ghasidas University, University of South Dakota
A one-pot practical, efficient, and environmentally benign multicomponent synthesis of 4H-pyrans and polysubstituted aniline derivatives of biological, pharmacological, and optical applications has been developed using a very mild, neutral, and reusable silica nanoparticles as catalyst. The 4H-pyran derivatives were synthesized by a three component reaction of an aldehyde, malononitrile, and 5,5-dimethyl-1,3-cyclohexanedione or ethyl acetoacetate at room temperature or refluxing in ethanol. Alternatively, polysubstituted anilines were synthesized via a four component reaction of an aldehyde, a ketone, and two equivalents of malononitrile in ethanol.
Mass spec analysis was carried out using an Advion expression Compact Mass Spectrometer (CMS).
This is one from a set of two application notes. In this application note, we determine the purity of collection of a small (0.2%) impurity in a concentrated sample mix by mass-directed fraction collection using supercritical fluid chromatography (SFC) coupled with the Advion expression Compact Mass Spectrometer (CMS).
For the other application note on the recovery and purification of two, three component standard mixtures, click here.
Advion co-authored with a major pharmaceutical partner to create two application notes highlighting the benefits of supercritical fluid chromatography (SFC) coupled to a compact mass spectrometer for mass directed fraction collection. These application notes conclude that mass‐directed fraction collection with a simplified passive splitter on the Preparative JASCO SFC coupled with expression CMS system increases purification selectivity by mass directed fraction collection while maintaining excellent recovery and purity. The MS accepted high concentrations of sample material with peak shapes trailing edges almost indistinguishable from the UV trace. Low, Middle and High isocratic flow rates all showed comparable MS source clearance leading to minimal tailing beyond the UV. Gradients further minimized peak tailing as expected on the UV and this was also seen on the TIC and XIC. Peak purities for all main band purifications were ≥99.9% pure and minor impurity collections showed equivalent success yielding 100% pure fractions. Acceptable recoveries for all collected fractions were 94% or better.