Routine detection and identification of PET radiopharmaceuticals

The rapid identification of radiolabeled compounds would clearly be beneficial for applied clinical and preclinical tracers used for imaging with positron emission tomography (PET). The limited uptake of mass spectrometry in the imaging field has been due to several factors including concerns regarding adequate sensitivity, instrument footprint in the highly constrained space requirements for the majority of labs and high initial capital costs plus high ongoing maintenance costs.

The introduction of the expression Compact Mass Spectrometer (CMS) at the 2012 Spring ACS National Meeting addressed for the first time three of the concerns: space, capital cost and maintenance costs. Only the question of adequate sensitivity remained to be answered.

Simple, high-performance coupling of SFC to CMS for mass-directed fraction collection

This is one from a set of two application notes. In this application note, we review recovery and purity determination of two, three component standard mixtures by supercritical fluid chromatography (SFC) couped with the Advion expression Compact Mass Spectrometer (CMS).

For the other application note on the fraction collection of a 0.2% impurity, click here.

Advion co-authored with a major pharmaceutical partner to create two application notes highlighting the benefits of supercritical fluid chromatography (SFC) coupled to a compact mass spectrometer for mass directed fraction collection. These application notes conclude that mass‐directed fraction collection with a simplified passive splitter on the Preparative JASCO SFC coupled with expression CMS system increases purification selectivity by mass directed fraction collection while maintaining excellent recovery and purity. The MS accepted high concentrations of sample material with peak shapes trailing edges almost indistinguishable from the UV trace. Low, Middle and High isocratic flow rates all showed comparable MS source clearance leading to minimal tailing beyond the UV. Gradients further minimized peak tailing as expected on the UV and this was also seen on the TIC and XIC. Peak purities for all main band purifications were ≥99.9% pure and minor impurity collections showed equivalent success yielding 100% pure fractions. Acceptable recoveries for all collected fractions were 94% or better.

Real-Time monitoring of a Suzuki reaction utilizing a hood-based compact mass spectrometer

A novel compact single quadrupole mass spectrometer for hood-based applications in the analysis of chemical reactions.

Medical chemists are routinely faced with personally over a hundred new chemical entities (NCE’s) each year for testing as future pharmaceutical drug candidates. The goal is to prepare a high yield, relatively pure product via an optimized synthetic route.