Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: The research in the Biosyn group is focused on the design, synthesis and function of the four major types of biomolecules: nucleic acids, carbohydrates, peptides and lipids and hybrid structures thereof. These biomolecules and their derivatives are used in drug discovery and chemical biology, to develop synthetic methodology or as an inspiration for mimetic design.
Q: WHAT WAS YOUR PREVIOUS EXPERIENCE?
A: I started as organic chemist on Pt-adducts on self-made DNA fragments under supervision of Professor Dr. J. Reedijk. Following that, I switched to synthesis of bio-organic molecules on solid support under supervision of Professor Dr. J.van Boom. Currently I am working with sophisticated analytical equipment including many (prep) LCMS systems under supervision of Professor Dr. H. Overkleeft. With this equipment we show our facility to many other workgroups within and outside the University of Leiden.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: It’s easy, walk-up use with straightforward swapping of ESI and APCI sources make it a reliable, productive tool for determining synthetic success.
Q: HOW DID THE EXPRESSION CMS HELP RESOLVE YOUR CHALLENGES?
A: I incorporated the expression CMS as TLC/MS system because of its simplicity, low cost and the brilliant combination with the Plate Express (as a TLC interface).
Q: TO WHOM WOULD YOU RECOMMEND THE EXPRESSION CMS?
A: I recommend the expression CMS to people who want to use a robust system with simple handling (like organic chemists).
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: The synthesis of modified histidines for solid-phase peptide synthesis and new routes to exocyclic allenes.
Q: WHAT WAS YOUR PREVIOUS WORK FLOW OR CHALLENGE?
A: Some Nim-tritylated histidine analogues and exocyclic allenes provided weak or no molecular ions using other mass spectrometry methods.
Q: WHY DID YOU INCORPORATE THE EXPRESSION® CMS INTO YOUR LABORATORY?
A: It’s easy, walk-up use with straight-forward swapping of ESI and APCI sources make it a reliable, productive tool for determining synthetic success.
Q: HOW DID THE EXPRESSION® CMS HELP RESOLVE YOUR CHALLENGES?
A: ESI and APCI resolved extensive fragmentation observed with some analytes.
Q: TO WHOM WOULD YOU RECOMMEND THE EXPRESSION® CMS?
A: Organic synthesis labs and undergraduate programs needing a simple diagnostic tool for reaction outcomes.
Q: WHAT IMPRESSES YOU THE MOST ABOUT THE EXPRESSION® CMS?
A: Students find it straight-forward to use and its sources are easy to clean.
The Drug Discovery Unit at the University of Dundee is met daily with a global task: address unmet medical needs and diseases in the developing world, and continuously build their innovative targets portfolio.
Learn from Susan Davis, a Medicinal Chemist with the unit, how she has processed 22,639 sample injections via the Advion compact mass spectrometer, and how it has assisted her research daily.
Also hear from Dr. Jack Henion, Advion’s Scientific Founder, as he joins for a Q&A session about mass spec and it’s role in drug discovery and disease prevention.
This webinar examines the workflow of a medicinal chemist as they utilize compact mass spectrometry techniques for drug discovery and disease prevention.
At regular intervals throughout the year a local lawn service applies pesticides to the lawn surrounding the Advion headquarters in Ithaca, NY. The grass is very uniform and not a single dandelion or crabgrass plant is visible – so we wondered, what chemical do they use? After an application in the early spring, we sampled the yellow pellets and utilized the Advion expression® CMS (compact mass spectrometer) coupled with a vapor Atmospheric Pressure Chemical Ionization (vAPCI) ion source to determine the unknown chemical.
In this application note, we demonstrate the use of the Advion Interchim Scientific expression® CMS with a volatile APCI (vAPCI) ion source to analyze extra-virgin, virgin, and lampante olive oil samples. We show statistical analysis allowing us to categorize and identify olive oil samples by their grade. This technique allows us to test individual olive oil samples and determine if they are truly extra-virgin, virgin, or lampante grade.
Cheese is one of the world’s most popular food types, with a wide variety available for consumers. The characteristic scents and flavors of cheeses stem from a complex mixture of chemicals, including free fatty acids. Use mass spectrometry to characterize the different volatile profiles of cheese.
In this application note, we demonstrate the capability of the Advion expression® Compact Mass Spectrometer (CMS) to analyze volatile fatty acids of various types of cheeses using volatile APCI (vAPCI). By heating the cheese samples, we released various volatile compounds and analyzed the headspace without any sample preparation or derivatization. We then performed statistical analysis to group the cheese samples by their volatile profiles.
Sudan Dyes are synthesized azo-dyes which are widely used to generate color in plastics, leather, fabrics, oil, waxes, etc. They are classified as Group 3 carcinogens by the International Agency for Research on Cancer (IARC) and are banned as food additives worldwide. However, in some countries these dyes are still occasionally used in order to intensify the color in products. In this note, a TLC/CMS method using expression® compact mass spectrometer (CMS) coupled with the Plate Express™ Interface is introduced for the detection of 7 Sudan dyes in a chili matrix.
In this application note, we demonstrate the use of the Advion expressionL CMS with an atmospheric solids analysis probe (ASAP) ion source to analyze two supplements (figure 1) that contain DMBA, a synthetic stimulant that has not been tested on humans. The substance is the chemical cousin to drug DBAA, which is currently banned by the US FDA. The efficacy and safety of DMBA is entirely unknown, yet it is present in many supplements.
The Montclair University team utilizes the Advion CMS in their undergraduate lab. Essential in the research-focused curriculum, the team was able to purchase the cost effective, small-footprint system to grow their lab capabilities. They later added an APCI module for their growing needs.
The Loughborough University team has utilized the expression® CMS vAPCI source for a variety of applications including breath analysis, monitoring flavor and fragrance, plant metabolomics, food authenticity and spoilage, and microbiological monitoring. With the use of the expression® CMS, the team was able to successfully execute detection required for these applications quickly and easily.
