Available On Demand, Original Air-Date, February 12, 2025 from Lab Manager
Coastal waters worldwide are contaminated with millions of metric tons of munitions from two world wars, posing significant risks to both ecosystems and human health. Traditional laboratory-based chemical methods for detecting munition compounds in seawater often require weeks or even months to process samples from collection to analysis.
As an attendee, you will learn more about:
- How real-time analysis can be performed utilizing novel sample collection and introduction paired with portable compact mass spectrometry
- Techniques to reduce errors, time, and labor involved in environmental sampling and analysis
- How operating a laboratory on a ship impacts workflow, from sample collection to analysis
Speaker: Aaron Beck, Ph.D.
Senior Scientist, GEOMAR
Helmholtz Centre for Ocean Research, Kiel, Germany
In this application note, we compare the use of a flash chromatography system with a UV detector alone versus the same system paired with a mass spectrometer. The data highlights the impact on workflow efficiency and underscores the importance of selecting the appropriate splitter.
The specificity of MS detection improves the efficiency of the purification workflow (compared to traditional Flash and HPLC).
In this application note, scientists from GEOMAR Helmholtz Centre for Ocean Research Kiel research residual TNT in the water phase from dumped weapons throughout the southwestern Baltic Sea. The Advion Interchim Scientific® expression® Compact Mass Spectrometer (CMS) is used for real-time sample detection of explosive chemicals released from these underwater relic munitions.
How it Works Webinar
Please join us for an insightful webinar where we’ll focus our discussion on the qualitative analysis of oligonucleotides using the HPLC-UV/MS method. Discover how this approach can offer you purity analysis, and molecular weight determination through charge deconvolution, etc., and gain access to valuable tricks and tips for optimizing oligonucleotide analysis. Additionally, learn how a streamlined method can provide both UV and MS analysis in a single run.
Speaker:
Changtong Hao
Senior Applications Scientist
Advion Interchim Scientific®
This Webinar was originally presented November 9, 2023 on LabRoots.com
ABSTRACT
In this webinar, you’ll learn how to address your lab’s purification pain-points with methods and instrumentation to maximize your budget, time and increase overall efficiency. During this session you will discover:
- How to select the ideal purification solution for your lab’s specific needs
- How to optimize your purification workflow daily, to automate compound ID and fraction confirmation – using simple, prep free tools
- How your cell phone can be used for automated method development
- How column selection factors in to your workflow, and why exploring what is best for your method matters
SPEAKER
Chase Needham, puriFlash and PrepLC Applications Scientist, Advion Interchim Scientific®
Oligonucleotides have gained significant attention in biopharmaceutical development due to their ability to modulate gene or protein expression. This application note aims to demonstrate the HPLC/UV analysis of multiple oligo samples and utilize HPLC/CMS analysis to determine their molecular weight.
In this application note, we use whey protein isolate and two casein macro peptides as an example to demonstrate the isolation and purification of bioactive peptide components with a system comprised of a puriFlash® 5.250 prepLC coupled online to an expression® CMS detector.
A book of experiments for your students
The expression® CMS (Compact Mass Spectrometer) is the ideal instrument for the teaching lab thanks to its small size, ease of use, and one-click software. This pocket guide of classroom experiments offers students the ability to gain hands-on instrument experience by eliminating complex and difficult sample preparation.
In this application note, we demonstrate a complete solution for the synthesis, purification, and quantitation of Gd(III)-DOTA, an ionic imaging contrast agent with better chemical stability and lower toxicity compared to the currently used Gd-(DTPA).
Iohexol is a widely used non-ionic imaging agent that improves contrast for x–ray analysis. Its low osmolality allows for a rapid clearance via the kidney, preventing reabsorption and further metabolization. This makes iohexol a compound with a better safety profile compared to other imaging agents.
In this application note, a simple and accurate HPLC-CMS method for iohexol analysis is introduced.
