The Advion expression Compact Mass Spectrometer (CMS) was coupled to a Normal Phase Flash Chromatography system for mass directed fraction collection. Typically a UV detector is used to monitor compounds eluting from Normal Phase columns. A limitation of the UV detector is that it relies on compounds having a chromophore in the chemical structure, hence an analyte of interest may not be detectable if it lacks a suitable UV chromophore or coelutes with another compound. In addition, solvent systems can interfere with and hinder UV absorbance. The Advion expression CMS provides the ultimate in selectivity, unambiguously identifying residual starting materials, desired product ions, side reactants and impurities by their molecular weight despite co-eluting compounds and solvent peaks. Mass directed fraction collection greatly speeds up the chemist’s work flow with confidence in the content and purity of the collected fractions by eliminating off-line TLCs, workup and core lab LC/MS assays.
Ultimate in Analytical Flexibility. Multi-Mode expression CMS
The expression CMS was developed with the chemist’s objectives and laboratory workflow in mind. Organic, Natural Product and Peptide chemists utilize many techniques to determine the progress, yield and purity of their reactions.
Mass spectrometry guided purification for efficient isolation of natural products at semi- and preparative scale
In natural product research the isolation of compounds from crude extracts is a key element. An increase in the efficiency of the purification process, by improvement of instrumental and methodological approaches, remains a crucial point. In this respect, modern flash chromatography, has evolved and uses 15 μm spherical particles for efficient separation at moderate pressures. Flash C-18 was tested for the rapid isolation of various plant secondary metabolites of interest.