Integrated Solutions for Food Authenticity, Characterization and Safety with the expression® Compact Mass Spectrometer, AVANT® (U)HPLC and SOLATION® ICP-MS
Your integrated laboratory solution for comprehensive testing of food, ingredients and natural products. Advion offers a full suite of analytical instrumentation for characterization, screening and purity determination, including:
- expression® CMS – The expression® Compact Mass Spectrometer (CMS) offers the industry’s widest range of sample introduction systems, providing rapid answers with little or no sample prep in < 30 seconds.
- SOLATION® ICP-MS – Provides fast, simple, multi-element analysis of toxic metals, and essential elements and minerals in a wide array of samples, including water, soil, plant material, food and raw ingredients
- AVANT® HPLC & UHPLC – Advion’s range of modular high performance, liquid chromatography systems can be used standalone with UV and UV/Vis detector options, or with the expression® CMS to provide seamlessly integrated LC/CMS under the full control of Advion’s simple, intuitive software suite.
- PLATE EXPRESS® TLC PLATE READER – Plate Express® provides a simple, automated means of obtaining mass spectra directly from TLC plates, combined with Advion’s expression® CMS creating a technique known as TLC/ CMS. See results in < 30 seconds at the push of a button.
Integrated Solutions for Potency and Safety with the expression® Compact Mass Spectrometer and SOLATION® ICP-MS
Outfit your lab with Advion Interchim Scientific’s line of custom cannabis solutions, including the expression® Compact Mass Spectrometer (CMS), the SOLATION® ICP-MS, and a customizable (U)HPLC solution.
Your Complete Lab Solution: Advion Interchim Scientific provides integrated laboratory solutions for comprehensive cannabis testing, including potency testing and cannabinoid levels as well as pesticide identification and quantitation of heavy metals.
For One-Touch Mass Analysis of Solids, Liquids, Surfaces and Fibers with the expression® CMS
The Touch Express™ Open Port Sampling Interface (OPSI), is designed for simple sampling of surfaces, solids, liquids and sample preparation tips and fibers. The novel ambient sampling technique was developed by Gary Van Berkel and Vilmos Kertesz, of Oak Ridge National Laboratory.
Paired with the electrospray ion source of the expression® Compact Mass Spectrometer, the solvent forms a meniscus at the open port before being drawn down the inner path into the electrospray ion source of the mass spectrometer under the Venturi effect of the nebulization gas. Any soluble sample touching the port is analyzed by the mass spectrometer in just seconds. Touch Express™ OPSI offers a fast assay bench-top solution for solid, liquids, and surfaces in a small-footprint, easy-to-use system.
Introduction
Electrospray mass spectrometry (ESI-MS) is a versatile analytical tool for biomolecular protein analysis and proteomics. One important area is to study protein structure and composition in which peptides and proteins can be ionized with multiple charges via electrospray ionization. Based on the charge envelope, the molecular weight can be easily determined using manual or automated deconvolution.
Touch Express™ is a new sampling device for mass spectrometry analysis using a technique developed by Gary Van Berkel and Vilmos Kertesz of Oak Ridge National Laboratory1 — the Open Port Sample Interface (OPSI). The OPSI incorporates an open port of continuous, low-volume solvent, flowing directly into the electrospray ion source of the mass spectrometer. Analysis is carried out by simply touching a sample to this port. This fast, versatile sample inlet paired with the expression® Compact Mass Spectrometer (CMS) provides simple molecular weight determination of surfaces, solids and liquids.
Touch Express™ OPSI is a direct analysis method for peptide and protein identification, introduced into the Advion expression® CMS and ionized via the ESI source. The sample can be analyzed < 1 min, and the continuous flow of solvent by OPSI also saves chemists more time for their compound analysis without contamination issues when one system is shared by multiple users or when running back-to-back samples. The deconvolution feature implemented in Advion Data Express software can easily help chemist to predict uncharged mass of their target peptides or proteins based on their charge envelope. Three compounds: BSA, myoglobin (horse) and lysozyme, are used for demonstration.
Quick and easy analysis methods for drugs of abuse are in high demand from health practitioners and law enforcement agencies on a global scale. In 2017, it was found that fentanyl, a fast-acting synthetic painkiller that is nearly 100 times stronger than morphine, has become the most widely abused synthetic opioid medicine – to the point it has become a public health problem. In this application note, a simple method for fentanyl screening in urine is introduced, using Advion Interchim Scientific’s Touch Express™ Open Port Sampling Interface (OPSI) coupled with the expressionL Compact Mass Spectrometer (CMS).
OPSI was developed by Gary Van Berkel and Vilnos Kertesz of Oak Ridge National Laboratory. Paired with the electrospray ionization (ESI) source of the CMS, Touch Express™ OPSI offers a fast assay benchtop solution in a small-footprint, easy-to-use system.
Introduction
Fentanyl, a fast-acting synthetic painkiller about 100 times stronger than morphine, is also the most widely used synthetic opioid medicine in 2017.1 Its abuse is becoming a public health problem. A quick and easy analysis method for this drug is in high demand from health practitioners and law enforcement agencies.
The Touch Express™ Open Port Sampling Interface (OPSI),2,3 is designed for simple sampling of solids, liquids, sample preparation tips and fibers. Paired with the ESI of the expression® CMS, the product incorporates an open port of continuous low-volume solvent, flowing directly into the ESI for MS analysis. Any soluble sample touching the open port will be analyzed by the mass spectrometer in just seconds.
Here, a simple and quick analysis method for fentanyl in urine using Touch Express™ OPSI on the Advion expression® CMS is presented.
The Touch Express™ Open Port Sampling Interface (OPSI), is designed for simple sampling of solids, liquids and sample preparation tips and fibers. The novel ambient sampling technique was developed by Gary Van Berkel and Vilmos Kertesz, of Oak Ridge National Laboratory.
Paired with the electrospray ion source of the expression® Compact Mass Spectrometer, the product incorporates a low volume, open port of continuously swept solvent, flowing directly into the electrospray ion source of the mass spectrometer.
During this webinar you will:
- Learn how any soluble material touching the port is analyzed by the expression® Compact Mass Spectrometer in just seconds.
- Learn how the sample technique eliminates sample preparation and provides zero carry over.
- Find fast analysis methods for solids, liquids and sample preparation tips and fibers.
Touch Express™ is a new sampling device for mass spec analysis using a technique developed by Gary Van Berkel and Vilmos Kertesz of Oak Ridge National Laboratory – the Open Port Sample Interface (OPSI). This fast, versatile sample inlet paired with the expression® Compact Mass Spectrometer (CMS) provides simple molecular weight determination of surfaces, solids and liquids in < 30s.
Gary Van Berkel, Vilmos Kertesz
Rationale
An “Open Access”‐like mass spectrometric platform to fully utilize the simplicity of the manual open port sampling interface for rapid characterization of unprocessed samples by liquid introduction atmospheric pressure ionization mass spectrometry has been lacking. The in‐house developed integrated software with a simple, small and relatively low‐cost mass spectrometry system introduced here fills this void.
Methods
Software was developed to operate the mass spectrometer, to collect and process mass spectrometric data files, to build a database and to classify samples using such a database. These tasks were accomplished via the vendor‐provided software libraries. Sample classification based on spectral comparison utilized the spectral contrast angle method.
Results
Using the developed software platform near real‐time sample classification is exemplified using a series of commercially available blue ink rollerball pens and vegetable oils. In the case of the inks, full scan positive and negative ion ESI mass spectra were both used for database generation and sample classification. For the vegetable oils, full scan positive ion mode APCI mass spectra were recorded. The overall accuracy of the employed spectral contrast angle statistical model was 95.3% and 98% in case of the inks and oils, respectively, using leave‐one‐out cross‐validation.
Conclusions
This work illustrates that an open port sampling interface/mass spectrometer combination, with appropriate instrument control and data processing software, is a viable direct liquid extraction sampling and analysis system suitable for the non‐expert user and near real‐time sample classification via database matching. Published in 2016. This article is a U.S. Government work and is in the public domain in the USA.
The OPSI/MS analysis was carried out using Advion Expression® CMS.
The Touch Express™ Open Port Sampling Interface (OPSI), is designed for simple sampling of solids, liquids and sample preparation tips and fibers. The novel ambient sampling technique was developed by Gary Van Berkel and Vilmos Kertesz, of Oak Ridge National Laboratory.
Paired with the electrospray ion source of the expression® Compact Mass Spectrometer, the product incorporates a low volume, open port of continuously swept solvent, flowing directly into the electrospray ion source of the mass spectrometer.
