In this application note, we show how Cyanidin-3-glucoside can be extracted from black rice and purified with a prepLC/Flash system. The amount and purity of Cyanidin-3-glucoside was measured utilizing a certified reference standard.
In this application note, a method to separate and purify green tea catechins with preparative liquid chromatography (Prep-LC) using the puriFlash® 5.250 system is demonstrated. An additional HPLC-UV-MS method is also demonstrated for compound confirmation and purity analysis.
In this application note, a method to separate and purify 3 curcuminoids from turmeric powder using thin layer chromatography (TLC) with mass spectrometry and flash chromatography is demonstrated.
In this application note, we describe methods of isolating natural products from green tea and turmeric powder by mass-directed fraction collection during flash chromatography and preparative LC. For demonstration purposes, the isolated compounds were then additionally confirmed by Atmospheric Solids Analysis Probe (ASAP®) MS or HPLC-MS.
The new edition of our “Analytical Sciences, Synthesis & Purification” catalog is available now! Offering a full suite of innovative products and technologies from Advion Interchim Scientific, this catalog features over 20,000 products ranging from consumables and reagents to instrumentation.
Not only a product resource, the catalog features in-depth scientific information to improve your daily workflow, including sample preparation tips and tricks, analysis technologies such as GC and LC/MS, and help selecting the best consumables for your workflow or purification.
Take advantage of this extensive guide to boost your lab with:
- 20,000 Products
- 12 Chapters
- Technicals Tips
- New Systems
Lab Manager Ask the Expert Webinar. Recorded September 15, 2022
Out of your element? Don’t be! This webinar features innovative cannabis and blood applications, plus tips and tricks to maximize throughput, precision, and sensitivity when utilizing ICP-MS technology. Performed using the Advion Interchim Scientific SOLATION® ICP-MS featuring a state-of-the-art sample introduction from Glass Expansion, this session is a must-see for any user or future user seeking tools for heavy metal analysis.
As a viewer, you will learn more about:
- How to perform heavy metal analysis of cannabis plants using the SOLATION® ICP-MS
- Simple methods for blood and plasma analysis that any lab can perform
- How spray chamber performance can affect long-term system stability
Webinar speakers include:
Dr. Fadi Abou-Shakra
Director of ICP-MS Portfolio
Advion Interchim Scientific
ICP-MS Application Scientist
Advion Interchim Scientific
Dr. Ryan Brennan
Cannabis and hemp products are becoming much more available for medicinal and recreational use making routine testing for toxic heavy metals much more important. Advion Interchim Scientific® introduces the SOLATION® ICP-MS for the analysis of heavy metals in cannabis plant and cannabis product samples. While there are no federal guidelines for heavy metals in cannabis, states where cannabis use and production are legal have adopted exposure limits and QC criteria for Arsenic, Cadmium, Mercury, and Lead based on USP<233>. Here, we report the results of our sample analysis using these guidelines.
This poster was presented at the 2021 ASMS conference in Philadelphia, PA, USA.
Flash chromatography has traditionally used UV absorption as the main method of detection for compounds during a purification process. While UV absorption is broadly applicable to many classes of compounds, it has limited specificity to individual compounds in a mixture and misses classes of compounds that do not carry chromophores.
Mass-directed fraction collection gives users the ability to collect fractions based on mass spectrometry detection (MS) which is based on ions specific to individual compounds and provides specific molecular information. This allows for simplification in the overall purification process and greater confidence in the identity of each isolated compound. Here we describe methods of isolating natural products from green tea and turmeric powder by mass-directed fraction collection during flash chromatography and preparative LC.
This poster was presented at the 2021 ASMS national conference in Philadelphia, PA.
In this exclusive webinar, our cannabis instrumentation experts will share information about solutions that relieve the purification bottleneck for processors.
This 15-minute on-demand session will cover:
- Pesticide remediation and dewaxing
- THC remediation
- Minor cannabinoid purification
- In-house analytical
- System and Consumable options
Authors: USDA-Agricultural Research Service, Agios Pharmaceuticals, TIC Gums, United States; Universidade Federal de Lavras, Brazil
Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this extract, prodigiosin and the serratamolide serrawetin W1, were identified based on mass and collision induced dissociation mass fragmentation spectra. An additional four compounds with M+H+ masses (487, 541, 543, and 571) consistent with serratamolides reported in the literature were also detected. Several other compounds with M+H+ masses of 488, 536, 684, 834, 906, and 908 m/z were detected in this ethanol extract inconsistently over multiple liquid chromatography coupled with tandem mass spectrometry (LC/MS–MS) runs. A purified preparation of prodigiosin provided control of damping-off of cucumber caused by P. ultimum when applied as a seed treatment while ethanol extract of cell mass of strain Tn246, a transposon-mutant-derivative of strain N4-5, did not. Strain Tn246 contained a mini-Tn5 Km insertion in a prodigiosin biosynthetic gene and was deficient in production of prodigiosin. All other compounds detected in N4-5 extract were detected in the Tn246 extract. This is the first report demonstrating that prodigiosin can control a plant disease. Other compounds in ethanol extract of strain N4-5 may contribute to disease control.
TLC/MS characterization of prodigiosin was performed using the Advion Interchim Scientific® Plate Express™ Automated TLC Plate Reader and expression® Compact Mass Spectrometer (CMS).