Quantitation of Perchlorate by Ion Chromatography with Suppressed Conductivity on the expression CMS
Perchlorate is a synthetic chemical that is used in the manufacture of rocket fuel, fireworks, flares and explosives, and can also be present in bleaches and some fertilizers [1]. The EPA has devised drinking water regulations for Perchlorate to minimize its impact on human health and the environment.
Achieving lower analytical detection limits for Perchlorate in environmental matrices such as drinking water allows regulatory bodies like the EPA make better informed decisions on the degree of contamination of an environmental system, as well as the remedial actions required to limit adverse health and environmental effects.
The expression Compact Mass Spectrometer (CMS) provides lower detection limits and greater selectivity for Perchlorate compared to a conductivity detector (CD). The typical detection limit of a CD is in the mid to high-ppb range, the CMS can detect < 20 ppt.
The expression® CMS provides essential compound information quickly and improves the chemist’s workflow. The Atmospheric Solids Analysis Probe (ASAP®) permits fast analysis of solid and liquid samples and is a simple, low cost alternative to LC/MS methods. This direct analysis probe method has been shown to be useful for the analysis of volatile and semivolatile compounds. ASAP® is available as a combined ASAP-APCI source, or existing APCI sources can be easily modified to become dual APCI-ASAP capable.
Register to download the full application note.
The expression CMS provides essential compound information quickly and improves the chemist’s workflow. The Atmospheric Solids Analysis Probe (ASAP®) permits fast analysis of solid and liquid samples and is a simple, faster and lower cost alternative to LC/MS methods. This direct analysis probe method has been shown to be useful for the analysis of volatile and semivolatile compounds. ASAP is available as a combined ASAP-APCI source, or existing APCI sources can be easily modified to become dual APCI-ASAP capable.
Register to download the full application note which includes the results of an example analysis.
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: My group specializes in developing new methods for pharmaceutical, metabolomic and security applications. In particular, this has involved the analysis of volatiles, either by direct analysis of breath, or through using thermal desorption to extract volatiles from solid or liquid matrices.
Q: WHAT WAS YOUR PREVIOUS WORK FLOW OR CHALLENGES?
A: Previously, we used a quadrupole time-of-flight instrument for volatile analysis. These instruments are not really designed with the analysis of small volatiles in mind and required preconcentration for breath analysis.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: The expression CMS offers excellent performance in the low m/z range and proved highly sensitive for determining volatile species in breath, requiring no preconcentration. Secondly, the instrument is transportable and can be deployed at the site of sampling giving high sample throughput and real-time measurements.
Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE EXPRESSION CMS?
A: Anyone interested in volatile analysis would find the CMS an excellent instrument for targeted analysis.
Via Direct Injection using a Compact Mass Spectrometer with extended mass range (CMSL)
The CMSL is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — ideal for large molecule applications such as the analysis of proteins. Its small footprint makes it the instrument of choice for space-restricted laboratories and is more affordable than comparable MS systems. The CMSL brings the analytical benefits of mass spectrometry to more scientists than ever before.
Register to download the application note and see how direct injection with the expression CMSL is a fast, powerful, yet simple tool to determine, confirm or characterize the mass of a relatively pure protein analyte.
Using the expression CMSL compact mass spectrometer
The CMSL is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — well-suited for large molecule applications such as the analysis of proteins. Its small footprint makes it the instrument of choice for space-restricted laboratories and it is more affordable than comparable MS systems. The CMSL brings the analytical benefits of mass spectrometry to more scientists than ever before.
Register to download the application note and see how LC/MS analysis using the expression CMSL and a denaturing solvent phase is a powerful tool in the analysis of protein mixtures and antibodies.
Q: What is the focus of your lab’s research?
A: Reach Separations is a contract research organization that services chemical, agricultural, pharmaceutical and academic clients.
Capability developed around expertise in separation science, specifically purification/prep.
Q: What previous workflow challenges did you experience?
A: Needed a tool to confirm quality of material (in and out).
Q: Why did you incorporate the expression CMS into your laboratory?
A: We selected the expression CMS for several reasons; proper MS Detector at an attractive price as well as its compact size because our lab space is limited. Advion’s technical support and service is exceptional. Easy to integrate with our wide range of equipment. Vendor relationship that is two way, Advion listens to our needs and ideas.
Q: Who Would you Recommend to purchase the expression CMS?
A: Anyone who needs a reliable mass spectrometer upgrade/addition for existing equipment.
Q: WHat is tHe focuS of your lab’S reSearcH?
A: The group has comprehensive experiences for undertaking radiotracer experiments, different spectroscopic techniques, characterization of colloid-chemical properties and in vitro/in vivo studies in view of the development and characterization of novel radiotracers, including functionalized nanoparticles. The design and development of chemically, metabolically and radiolytically stable radiometal complexes on the basis of 64Cu/67Cu is of special interest. Furthermore, polynuclear metal compounds (polyoxometalates, Re clusters) for anti-tumor activity, photosensitizing, and radiation-sensitizing properties are under investigation. The characterization of binding and transport behavior of novel receptors by solvent extraction studies represents one main topic.
Q: WHAT PREVIOUS WORKFLOW CHALLENGES DID YOU EXPERIENCE?
A: The development of new bifunctional chelating agents and relevant target-seeking bio-conjugates requires novel synthetic strategies and the improvement of known pathways as well. For the successful achievement of this goal, ‘real-time’ characterization of main and side-products is of particular importance.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: The Advion expression compact mass spectrometer rapidly provides reliable mass data in a stand-alone modus and coupled to UPLC/HPLC systems, respectively. Sample handling and measurement are straightforward. PhD students appreciate both the ‘in-time’ information about success and failure in developing novel compounds. I recommend to all groups requiring immediate access to reliable mass data for the characterization of novel compounds.
Q: What is the focus of your lab’s research?
A: The mass spectrometry facility here at Boston College’s main focus is as a core lab, open to all users, both undergraduate and graduate students alike.
Our main research interests include the application of mass spectrometry as a routine tool for chemistry/chemical biology; exploring new research applications for mass spectrometry; and the use of open-air ionization methods for the analysis of pharmaceutical, biological, drug and chemical analysis.
Q: What was your previous work flow or challenges?
A: As a core lab we have a large number of samples being run and there is a constant need to change ionization sources.
Q: Why did you incorporate the expression® CMS into your laboratory?
A: The expression® has allowed us to take the workload of the other more expensive instruments by incorporating it as an open access instrument. With minimal training, the students are able to run their own samples, as not to slow down their research.
Due to the instrument size and speed at which it pumps down, we are able to move the instrument directly to the classroom for students to use.
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: During conventional lipodomics research, information is lost during the extraction when lipids get dissociated from the lipoprotein particles. We are developing methods to gather information about lipoproteins while looking at the lipid through online liquid/liquid extraction using the expression CMS as the detector.
Q: WHAT PREVIOUS WORKFLOW CHALLENGES DID YOU EXPERIENCE?
A: The previous method of fraction collection took a lot of time and a lot of work.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: The CMS is a rugged instrument and allows us to manipulate liquid/liquid extractions and modify parameters such as salt buffers. It is a very simple to use system. Other mass spectrometers are complicated, so if something goes wrong in the method development process; it takes a lot of effort to get the system back up and running. The CMS is easy to clean and can be back running in a short amount of time.
Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE EXPRESSION CMS?
A: I recommend the CMS to anyone interested in method development especially those that are not easily amenable to mass spectrometry, because it is so easy to run and maintain.
