In this study, total saponins were extracted from Pleurotus ostreatus cultivated with Astragalus as one of organic culture substrates. High Performance Thin Layer Chromatography (HPTLC) assay showed total saponins could be separated effectively, and four kinds of spots were identified as AG I, AG II, AG III, and AG IV, respectively. FTIR spectra based on HPTLC separation assay showed the saponin characteristic groups including ‐OH, C‐H, C=O, and the glycoside linkaged to sapogenin group C‐O‐C, suggesting the four kinds of spots belonged to cycloartane‐type triterpene saponins. The primary mass spectra of precursor ion (HPTLC‐ESI‐MS) assay further proved the main composition of four kinds of spots was AG I‐IV, respectively. Physical properties, including the detection of specific rotation and melting point, revealed the separation of high‐purity saponin monomer by HPTLC. HPTLC‐dual wavelength spectrodensitometric method detection showed that content of astragaloside I‐IV was ranged from 0.2 to 0.5 mg/g, and the total astragalosides contents attained to 1.397 mg/g, indicating P. ostreatus could bioaccumulate astragalosides from Astragalus. These results demonstrated the characterization of astragalosides based on the separation of HPTLC was effective, and supported to consider astragalosides‐enriched P. ostreatus as functional edible fungus for food and medical applications.
Practical Application
Currently, the consumption of enriched foods has become common and continues to increase due to urgent demanding for foods with high nutritional value. Pleurotus ostreatus is a functional edible fungus, which not only can produce secondary metabolites, but can enrich bioactive ingredients. Astragalosides have a wide range of biological activities, especially currently being tested as cardioprotective agent. In this study, P. ostreatus was cultivated through adding Astragalus into culture substrates, which realized massive enrichment of astragalosides. Astragalosides‐enriched P. ostreatus as functional edible fungus could be extensively used in food and medical areas, especially for the prevention of cardiovascular diseases.
Analysis was performed by LC/MS using the Advion expression Compact Mass Spectrometer (CMS).
University of Oklahoma Health Sciences Center, Christian Medical College, Veterans Administration Medical Center, University of Montreal
Abstract
A major source of epilepsy is Neurocysticercosis (NCC), caused by Taenia solium infection. Solitary cysticercus granuloma (SCG), a sub-group of NCC induced epilepsy, is the most common form of NCC in India. Current diagnostic criteria for SCG epilepsy require brain imaging which may not be available in communities where the disease is endemic. Identification of serum changes and potential biomolecules that could distinguish SCG epilepsy from idiopathic generalized epilepsy (IE), without the initial need for imaging, could assist in disease identification, understanding, and treatment. The objective here was to investigate, using mass spectrometry (MS), sera biomolecule differences between patients with SCG epilepsy or IE to help distinguish these disorders based on physiological differences, to understand underlying phenotypes and mechanisms, and to lay ground work for future therapeutic and biomarker analyses.
Analysis was performed by ESI ionization using the Advion Interchim Scientific® expression® Compact Mass Spectrometer (CMS).
Mass spectrometry is an analytical technique used to determine the mass-to-charge ratio of ions in a sample and therefore the sample composition. This method is used across many industries including food and beverage, environmental monitoring, and clinical. There are many features of mass spectrometers that must be considered before purchasing such as the required mass analyzer technology, ionization source, and dissociation technique. Join Lab Manager and our panel of experts as we discuss how to decipher mass spectrometry technology offerings and find the right solution for your lab.
As an attendee, you will learn more about:
Available technology for performing mass spectrometry
Features to consider when purchasing mass spectrometry instruments
How to find the right mass spectrometer for your application
Environmental monitoring is vital to understanding the world around us. Air quality, water, and soil testing all provide valuable information about an environment’s status. Tools and technologies for environmental monitoring can be used to evaluate the sanitation of a food and beverage lab or to monitor an ecosystem’s recovery after an oil spill. Join Lab Manager and our panel of experts as we review the tools available for environmental testing and monitoring.
As an attendee, you will learn more about:
Developments in technology for environmental testing and monitoring
Considerations when purchasing technology for environmental testing and monitoring
Applications for environmental testing and monitoring across industries
This webinar was hosted by Lab Manager Tech Trends and recorded July 9, 2020.
Lab Manager Tech Trends Webinar. Recorded June 25, 2020.
Food and beverage labs use techniques from other disciplines such as microbiology, environmental monitoring, and analytical chemistry to test for food and beverage contaminants. Unlike these disciplines, the food and beverage industry is highly regulated and therefore food and beverage labs have very specific needs. Join Lab Manager and our panel of experts as we discuss tools for food and beverage testing.
In this webinar, you will learn more about:
The latest technology for testing in the food and beverage lab
Applications for tools and technologies in the food and beverage lab
Considerations when purchasing instruments for food and beverage testing
Pacific Northwest National Laboratory, Clemson University
Abstract
A new, multi-electrode, liquid sampling glow discharge ionization source for mass spectrometry is described. This ion source consists of multiple (2–4) counter (anode) electrodes in comparison to prior single counter electrode designs of this type. In the experiments presented here these ion sources have been interfaced with ThermoScientific Exactive Orbitrap instruments and Advion Interchim Scientific® expression® Compact Mass Spectrometer (CMS) instruments. Advantages and analytical performance improvements are described. These include the ability to use higher plasma currents, resulting in a more robust and energetic plasma exhibiting higher sensitivity, lower spectral background, ppt detection limits, and 2–3× faster washout times. A low-cost, 3D printed version of a dual counter electrode design is also described. The ion source can further be utilized in either atomic (elemental/isotopic) or molecular (molecular ion, fragmentation) ionization modes.
This is one from a set of two application notes. In this application note, we determine the purity of collection of a small (0.2%) impurity in a concentrated sample mix by mass-directed fraction collection using supercritical fluid chromatography (SFC) coupled with the Advion expression Compact Mass Spectrometer (CMS).
For the other application note on the recovery and purification of two, three component standard mixtures, click here.
Advion co-authored with a major pharmaceutical partner to create two application notes highlighting the benefits of supercritical fluid chromatography (SFC) coupled to a compact mass spectrometer for mass directed fraction collection. These application notes conclude that mass‐directed fraction collection with a simplified passive splitter on the Preparative JASCO SFC coupled with expression CMS system increases purification selectivity by mass directed fraction collection while maintaining excellent recovery and purity. The MS accepted high concentrations of sample material with peak shapes trailing edges almost indistinguishable from the UV trace. Low, Middle and High isocratic flow rates all showed comparable MS source clearance leading to minimal tailing beyond the UV. Gradients further minimized peak tailing as expected on the UV and this was also seen on the TIC and XIC. Peak purities for all main band purifications were ≥99.9% pure and minor impurity collections showed equivalent success yielding 100% pure fractions. Acceptable recoveries for all collected fractions were 94% or better.
A simple, sensitive and selective ASAP® sample introduction approach to measuring the presence of two isobaric compounds, CBDA and THCA, contained in a complex sample such as hemp or cannabis plants or their corresponding extraction products. Measurements are made of differences in the relative composition of CBDA and THCA fragment ions originating from the same precursor ion. Applicability to screening plants and plant product materials such as hemp or marijuana to monitor out-of-specification composition is demonstrated.
This application was developed from the poster presented at the 2018 Cannabis Science Conference in Portland, OR.
The combination of the Advion Interchim Scientific expression® CMS and puriFlash®flash chromatography technologies speed up the synthesis, purification and characterization of a compound.
Using the Advion Interchim Scientific expression®Compact Mass Spectrometer, the Plate Express™ TLC Plate reader, the ASAP® Atmospheric Solids Analysis Probe, and the puriFlash®XS520 flash purification system, it is now possible to execute a fast, user-friendly workflow to run TLC, harness the power of your cell phone to develop a flash method, and finally utilize a simple dipping probe to confirm fractions in seconds.
With easy sampling techniques, intuitive software, and advanced detection technology, you can easily and confidently synthesize, purify and characterize your compound, in a flash.
The collection of solid particulates and liquids from surfaces by the use of cloth swipes is fairly ubiquitous. In such methods, there is a continuous concern regarding the ability to locate and quantitatively sample the analyte species from the material. In this effort, we demonstrate the initial coupling of an Advion Plate Express plate reader to a liquid sampling–atmospheric pressure glow discharge (LS-APGD) microplasma ionization source with an Orbitrap mass spectrometer to perform uranium isotopic analyses of solution residues on cotton swipes. The Plate Express employs a sampling probe head to engage and seal against the swipe surface. Subsequentially, the analyte residues are desorbed and transported within a 2% HNO3 electrolyte flow to the ionization source. Quantitative recoveries were observed following a single 30 s extraction step, with the absolute mass sampled per extraction being ∼100 ng. While the intrasample variability in the analytical responses for triplicate sampling of the same swipe yield ∼30% RSD, this lack of precision is offset by the ability to determine isotope ratios for enriched uranium specimens with a precision of better than 10% RSD. Pooled, intersample precision (n = 9) was found to be <5%RSD across the various sample compositions. Finally, 235U/238U determinations (ranging from 0.053 to 1.806) were accurate with errors of <10%, absolute. The 234U- and 236U-inclusive ratios were determined with similar accuracy in enriched samples. While the driving force for the effort is in the realm of nuclear nonproliferation efforts, the ubiquitous use of cloth swipes across many application areas could benefit from this convenient approach, including the use of versatile, reduced-format mass spectrometer systems.
