Developed in collaboration with Oak Ridge National Laboratory*, the liquid extraction surface analysis (LESA) capability of the TriVersa Nanomate® enables simple, direct ESI mass spectrometric analysis from a variety of surfaces.
Fully characterize oligosaccharides that are missed by LC/MS/MS.
Download this product note to find out how.
L. Tomlinson; J. Fuchser; A. Futterer; M. Baumert; D.G. Hassall; A. West; P.S. Marshall Rapid Commun. Mass Spectrom. 2014, 28, 995-1003
Automated static nano-ESI/MS/MS (Infusion MS/MS) is an easy-to-use and high-throughput approach to analyte identification. It is particularly useful in the field of shotgun lipidomics, which itself has great potential for biomarker discovery and gained some significant attention in the last couple of years (Schuhmann et al. 2012; Jung et al. 2011; Han et al. 2011).
In order to simplify shotgun lipidomic approaches even further, the TriVersa NanoMate can now change nano-ESI spray polarity within the same infusion experiment.
In many cases of routine protein identification, processing times become the major bottleneck at the core facility. Although a nano-LC/MS/MS approach is recognized as a general strategy for protein identification, the typical 30-120 min run-time prohibits fast turnaround times. The TriVersa NanoMate offers a more rapid approach for the infusion of the protein digest, with only a 2-5 min run-time per sample.
Presented by: Dr. Tom Covey
In this web presentation Dr. Tom Covey describes how researchers can incorporate the fast, simple & direct method of LESA Clarity for AB SCIEX Mass Spec Systems into their labs. He shows data that illustrates how you can see more analytes with better sensitivity.
Presented by: John P. Shockcor, Director of Life Sciences Business Development, Waters Corp., Visiting Fellow, Dept. of Biochemistry, University of Cambridge, UK
Description: Profiling low level components in a complex mixture of small molecules can be a challenging task. Although it may be possible to detect many low level components in a complex mixture, characterization is often hindered because fragmentation of these low level components yields peaks below the limit of detection. This problem can be alleviated by using a TriVersa NanoMate assisted approach. In this webinar we will describe how a TriVersa NanoMate coupled to a SYNAPT G2 Hybrid QTof Ion-Mobility Mass Spectrometer can provide critical fragmentation information needed to characterize low level components in lipidomics, drug metabolism studies and natural product profiling. This approach is ideally suited to the use of time-aligned-parallel fragmentation (TAP) which are illustrated by a number of examples.
Presented by: Dr. Daniel Eikel, Sr. Application Scientist – Advion, Inc.
Description: Liquid extraction surface analysis mass spectrometry (LESA-MS) is a novel mass spectrometry-based surface-profiling technique (Kertez, et al., 2009) that can be utilized in drug distribution and metabolism studies. Potential advantages of LESA-MS are as follows:
In this webinar, Dr. Daniel Eikel expands upon his publication (Henion, et al., 2011), discussing how LESA-MS can provide complementary information to the gold standards in this field: quantitative whole body autoradiography (QWBA) and whole organ LC-MS/MS, or other MS based approaches such as MALDI-MSI. He demonstrates how they have evaluated LESA-MS by studying the drug distribution and metabolism of terfenadine in mouse, and through direct comparison, proved that LESA-MS appears to be more informative than a comparable MALDI-MSI experiment and reflects the literature-known metabolism and distribution pattern for terfendine and its metabolite fexofenadine.
Presented by: Thomas Covey, Principal Research Scientist at AB SCIEX
Thomas Covey describes how various organizations are incorporating the fast, simple, and direct method of LESA – Liquid Extraction Surface Analysis – into their labs. He shows data that illustrates how you can see more analytes with better sensitivity by combining LESA with mass spectrometry and chip-based nanoESI via Advion’s TriVersa NanoMate. This new analytical technique has a wide range of applications for sample analysis from a variety of surfaces e.g. tissue slices, TLC plates, MALDI plates, and Dried Blood Spots.
Dr. Covey also demonstrates the latest LESA upgrade – LESA Points software that provides simple, point-and-click surface analysis. Users can scan a digital image of their samples and select sampling positions within 90-μm resolution. The new upgrade also provides a specially-developed mounting block that keeps all kinds of surfaces at a fixed height for simple method set-up.
In this webinar, Dr. Han describes how he uses the TriVersa NanoMate’s chip-based nanoelectrospray ionization capabilities in infusion mode to obtain more information from complex samples and faster lipids analysis with no sample-to-sample carryover. The interests of Dr. Han’s laboratory have been focused on the altered lipid metabolism, trafficking, and homeostasis under patho(physio)logical conditions. Currently, there are three specific areas explored in his laboratory including (1) extension of the shotgun lipidomics technology for increased penetrance into the low abundance regime of a cellular lipidome with emphasis on high throughput, and bioinformatics; (2) investigation of the biochemical mechanisms underlying the altered lipid content and composition in metabolic syndrome; and (3) identification of the biochemical mechanisms responsible for the sulfatide depletion and ceramide elevation at the very earliest stages of Alzheimer’s disease.
Presented by: Xianlin Han, Ph.D., Professor, Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute