“We have the expression linked to an HPLC instrument and it is used as an open access system. The combination of UV purity data along with MS confirmation is incredibly powerful for our chemists. The instrument is networked so our chemists can then process and review data from their desks.”
DYESTUFF ANALYSIS OF CULTURAL HERITAGE OBJECTS by Direct Analysis Probe on a Compact Mass Spectrometer
The scientific investigation of cultural heritage objects is the only access to knowledge about long-gone, ancient societies and their cultural, technological as well as their social stage of development. In addition, the materials used by our ancestors, e.g. their textiles and the organic dyestuff to colorize these materials, allow researchers to draw some conclusions about cultural exchanges and trading routes. But in-depth knowledge about materials and dyestuff is also of highest importance for proper conservation and restauration of these materials. Specimens, however, are rare and available only in minute amounts. For their investigation preferentially non-destructive or at least minimal invasive methods have to be used.
Compact Mass Spectrometry (CMS) for the Detection and Quantification of Chemical Compounds Related to Cannabis
The cannabinoid makeup of cannabis has significantly changed over the past few decades. Efforts to increase the psychotropic effects of cannabis have resulted in a THC content for current strains of the crop of 30 to 40 percent compared with only 5 percent 30 years ago. Up-regulating the THC content of the plant causes a loss of other cannabinoids the plant is producing. Some of these other cannabinoids, such as CBD, are believed to be responsible for its medical benefits.
Direct Mass Analysis of TLC Plates
Plate ExpressTM is a device that provides a simple, automated means of obtaining mass spectra directly from TLC plates, creating a technique known as TLC/MS. Combined with Advion’s expression® compact mass spectrometer (CMS) this becomes TLC/CMS. Using this technique chemists can quickly and confidently identify products even in complex mixtures without additional sample preparation.
NATURAL PRODUCT ANALYSIS Using Compact Mass Spectrometry with Polarity Switching and In-source CID
Natural products, the underlying active components and their use in traditional medicine are of increased interest in the field of pharmaceutical research. The naturally occurring active ingredients are ideal chemical starting structures to improve upon in the pharmaceutical drug development process and consequently half of all currently approved drugs in the US are based on natural products. It is therefore not surprising that natural product analysis is a field of major interest world wide. Here, we present a simple example of the added benefits of mass spectrometry in typical natural product analysis workflows based on an alcoholic extract of nutmeg.
SUZUKI REACTION MONITORING Using Compact Mass Spectrometry with TLC Interface
This application note presents an online thin-layer chromatography (TLC)/CMS technique using the Advion expression compact mass spectrometer (CMS) and Plate ExpressTM interface to provide compound structural information without sample preparation after TLC separation. A Suzuki reaction for the synthesis of 4-aminobiphenyl will be demonstrated. The online TLC/CMS technique provides rapid and accurate determination of reaction mixture components without the need for off-line TLC sample preparation procedures.
THC PLANT MATERIAL ANALYSIS Using Compact Mass Spectrometry for Product Control and Law Enforcement of cannabis-related Plant Products
Analysis of the active components of the cannabis sativa plant is required both for law enforcement where these compounds are illegal and also as a tool for product control and optimization in the increasing number of legal markets for cannabis plant products. Whenever simple, unequivocal and legally defensible analysis methods for the detection and quantification of plant metabolites are required, mass spectrometry is the detector of choice.
SUDAN DYES ANALYSIS Using Compact Mass Spectrometry with TLC Interface
Sudan Dyes are synthesized azo-dyes which are widely used to generate color in plastics, leather, fabrics, oil, waxes, etc. They are classified as Group 3 carcinogens by the International Agency for Research on Cancer (IARC) and are banned as food additives worldwide. However, in some countries these dyes are still occasionally used in order to intensify the color in products. In this note, a TLC/CMS method using expression compact mass spectrometer (CMS) coupled with the Plate ExpressTM Interface (Advion, Ithaca, NY) is introduced for the detection of 7 Sudan dyes in a chili matrix. The primary advantage of TLC/CMS analysis is the ability to sample directly from the TLC plate without further sample preparation after the TLC separation is done. Molecular weight information of sample components can be determined by the mass spectra from the eluted TLC spots.
expression CMS: Compact. Fast. Easy. & Affordable
With over 20 years of mass spectrometry and chemistry expertise, Advion has produced a family of compact mass spectrometers designed for the chemist. The affordability, small size and ease of use make them ideal for use directly at the chemist’s bench, giving immediate answers and informed decisions instead of waiting.
Expanded application note. DIRECT SAMPLE ANALYSIS: Detecting THC/Cannabinoids in Contraband by Compact Mass Spectrometry
This is an expanded application note with more data regarding direct sample analysis of THC in suspected contraband and on skin. Direct sample analysis is possible via an Atmospheric Solids Analysis Probe (ASAP®) combined with atmospheric pressure chemical ionization (APCI) on a compact mass spectrometer (CMS). This technique allows for the rapid investigation (<30 sec) of material suspected of containing THC/cannabinoids.