This whitepaper outlines an integrated and streamlined workflow for early-stage drug discovery, covering all key elements of drug development, including compound synthesis, reaction monitoring, purification, compound identification, and evaporation. By leveraging a seamless, end-to-end platform, pharmaceutical companies can accelerate lead optimization, reduce costs, and improve decision-making in the discovery process.
Advanced Arsenic Speciation In Water Using HPLC-ICP-MS
Inorganic arsenic, a highly toxic form of arsenic commonly found in water, can pose serious health risks. Accurate detection and quantification are essential for water safety and regulatory compliance. HPLC-ICP-MS offers a powerful solution by separating arsenic species and detecting them with high sensitivity.
The Advion Interchim Scientific® speciation platform enhances this workflow with integrated control of the SOLATION® ICP-MS and AVANT® HPLC systems, real-time UV-DAD data visualization, and advanced quantitation features like speciated isotope dilution. With simplified data review, flexible reporting, and automated column switching, it streamlines method development and boosts efficiency for multi-element analysis.
The Power of Precision: Navigating Qualitative Oligonucleotide Analysis with HPLC-UV/MS
How it Works Webinar
Please join us for an insightful webinar where we’ll focus our discussion on the qualitative analysis of oligonucleotides using the HPLC-UV/MS method. Discover how this approach can offer you purity analysis, and molecular weight determination through charge deconvolution, etc., and gain access to valuable tricks and tips for optimizing oligonucleotide analysis. Additionally, learn how a streamlined method can provide both UV and MS analysis in a single run.
Speaker:
Changtong Hao
Senior Applications Scientist
Advion Interchim Scientific®
Qualitative Analysis of Oligonucleotides Using The Advion Interchim Scientific® HPLC-UV/MS System
Oligonucleotides have gained significant attention in biopharmaceutical development due to their ability to modulate gene or protein expression. This application note aims to demonstrate the HPLC/UV analysis of multiple oligo samples and utilize HPLC/CMS analysis to determine their molecular weight.
Purification of Bioactive Peptides from Complex Mixtures using the puriFlash® 5.250 PrepLC and expression® CMS Detector
In this application note, we use whey protein isolate and two casein macro peptides as an example to demonstrate the isolation and purification of bioactive peptide components with a system comprised of a puriFlash® 5.250 prepLC coupled online to an expression® CMS detector.
The Preparation, Confirmation, and Quantitation of the Ionic Imaging Contrast Agent, Gd(III)-DOTA
In this application note, we demonstrate a complete solution for the synthesis, purification, and quantitation of Gd(III)-DOTA, an ionic imaging contrast agent with better chemical stability and lower toxicity compared to the currently used Gd-(DTPA).
Analysis of Iohexol using the Advion Interchim Scientific® AVANT® HPLC and expression® CMS System
Iohexol is a widely used non-ionic imaging agent that improves contrast for x–ray analysis. Its low osmolality allows for a rapid clearance via the kidney, preventing reabsorption and further metabolization. This makes iohexol a compound with a better safety profile compared to other imaging agents.
In this application note, a simple and accurate HPLC-CMS method for iohexol analysis is introduced.
The Extraction, Identification, Purification and Quantitation of Cyanidin-3-glucoside in Black Rice
In this application note, we show how Cyanidin-3-glucoside can be extracted from black rice and purified with a prepLC/Flash system. The amount and purity of Cyanidin-3-glucoside was measured utilizing a certified reference standard.
Extraction, Identification and Purification of (-)- Epigallocatechin Gallate From Green Tea
In this application note, a method to separate and purify green tea catechins with preparative liquid chromatography (Prep-LC) using the puriFlash® 5.250 system is demonstrated. An additional HPLC-UV-MS method is also demonstrated for compound confirmation and purity analysis.
Cannabinoid Analysis in Edible Samples Using the Advion Interchim Scientific AVANTTM UHPLC/PDA System
In this application note, a simple, cost-effective UHPLC/UV method is introduced for analysis of cannabinoids in edible samples (examples shown are gummy bears, chocolates and cookies) using the Advion Interchim Scientific AVANT™ UHPLC/PDA System.