Via Direct Injection using a Compact Mass Spectrometer with extended mass range (CMSL)
The CMSL is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — ideal for large molecule applications such as the analysis of proteins. Its small footprint makes it the instrument of choice for space-restricted laboratories and is more affordable than comparable MS systems. The CMSL brings the analytical benefits of mass spectrometry to more scientists than ever before.
Register to download the application note and see how direct injection with the expression CMSL is a fast, powerful, yet simple tool to determine, confirm or characterize the mass of a relatively pure protein analyte.
Using the expression CMSL compact mass spectrometer
The CMSL is a high performance, easy to use, single quadrupole mass spectrometer with an extended mass range analysis capability to m/z 2000 — well-suited for large molecule applications such as the analysis of proteins. Its small footprint makes it the instrument of choice for space-restricted laboratories and it is more affordable than comparable MS systems. The CMSL brings the analytical benefits of mass spectrometry to more scientists than ever before.
Register to download the application note and see how LC/MS analysis using the expression CMSL and a denaturing solvent phase is a powerful tool in the analysis of protein mixtures and antibodies.
Q: What is the focus of your lab’s research?
A: Reach Separations is a contract research organization that services chemical, agricultural, pharmaceutical and academic clients.
Capability developed around expertise in separation science, specifically purification/prep.
Q: What previous workflow challenges did you experience?
A: Needed a tool to confirm quality of material (in and out).
Q: Why did you incorporate the expression CMS into your laboratory?
A: We selected the expression CMS for several reasons; proper MS Detector at an attractive price as well as its compact size because our lab space is limited. Advion’s technical support and service is exceptional. Easy to integrate with our wide range of equipment. Vendor relationship that is two way, Advion listens to our needs and ideas.
Q: Who Would you Recommend to purchase the expression CMS?
A: Anyone who needs a reliable mass spectrometer upgrade/addition for existing equipment.
Q: WHat is tHe focuS of your lab’S reSearcH?
A: The group has comprehensive experiences for undertaking radiotracer experiments, different spectroscopic techniques, characterization of colloid-chemical properties and in vitro/in vivo studies in view of the development and characterization of novel radiotracers, including functionalized nanoparticles. The design and development of chemically, metabolically and radiolytically stable radiometal complexes on the basis of 64Cu/67Cu is of special interest. Furthermore, polynuclear metal compounds (polyoxometalates, Re clusters) for anti-tumor activity, photosensitizing, and radiation-sensitizing properties are under investigation. The characterization of binding and transport behavior of novel receptors by solvent extraction studies represents one main topic.
Q: WHAT PREVIOUS WORKFLOW CHALLENGES DID YOU EXPERIENCE?
A: The development of new bifunctional chelating agents and relevant target-seeking bio-conjugates requires novel synthetic strategies and the improvement of known pathways as well. For the successful achievement of this goal, ‘real-time’ characterization of main and side-products is of particular importance.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: The Advion expression compact mass spectrometer rapidly provides reliable mass data in a stand-alone modus and coupled to UPLC/HPLC systems, respectively. Sample handling and measurement are straightforward. PhD students appreciate both the ‘in-time’ information about success and failure in developing novel compounds. I recommend to all groups requiring immediate access to reliable mass data for the characterization of novel compounds.
Q: What is the focus of your lab’s research?
A: The mass spectrometry facility here at Boston College’s main focus is as a core lab, open to all users, both undergraduate and graduate students alike.
Our main research interests include the application of mass spectrometry as a routine tool for chemistry/chemical biology; exploring new research applications for mass spectrometry; and the use of open-air ionization methods for the analysis of pharmaceutical, biological, drug and chemical analysis.
Q: What was your previous work flow or challenges?
A: As a core lab we have a large number of samples being run and there is a constant need to change ionization sources.
Q: Why did you incorporate the expression® CMS into your laboratory?
A: The expression® has allowed us to take the workload of the other more expensive instruments by incorporating it as an open access instrument. With minimal training, the students are able to run their own samples, as not to slow down their research.
Due to the instrument size and speed at which it pumps down, we are able to move the instrument directly to the classroom for students to use.
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: During conventional lipodomics research, information is lost during the extraction when lipids get dissociated from the lipoprotein particles. We are developing methods to gather information about lipoproteins while looking at the lipid through online liquid/liquid extraction using the expression CMS as the detector.
Q: WHAT PREVIOUS WORKFLOW CHALLENGES DID YOU EXPERIENCE?
A: The previous method of fraction collection took a lot of time and a lot of work.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: The CMS is a rugged instrument and allows us to manipulate liquid/liquid extractions and modify parameters such as salt buffers. It is a very simple to use system. Other mass spectrometers are complicated, so if something goes wrong in the method development process; it takes a lot of effort to get the system back up and running. The CMS is easy to clean and can be back running in a short amount of time.
Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE EXPRESSION CMS?
A: I recommend the CMS to anyone interested in method development especially those that are not easily amenable to mass spectrometry, because it is so easy to run and maintain.
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: The central activity of our group is to design and develop molecules to study and control biological systems. We are using organic and organometallic synthesis to construct new bioactive compounds, new agents for bioconjugation, and new probes for biology. Our focus is to apply chemistry to biology by identifying targets and then creating cancer treatment strategies and therapy options.
Q: WHAT PREVIOUS WORKFLOW CHALLENGES DID YOU EXPERIENCE?
A: For identification and analysis, we use NMR and HPLC regularly, but had no mass spectrometer in the laboratory, so we gave our samples to outside MS service for analysis. Therefore, we had to wait for the results.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: Advion’s CMS allows us to have a self-service option. One ingenieur is in-charge of the CMS, but everyone can use it including students after a short formation. And for 80% of our work it is perfect. We still use the MS facility for more demanding analysis, but this new workflow speeds up our process significantly.
Q: WHO WOULD YOU RECOMMEND TO PURCHASE THE EXPRESSION CMS?
A: I would recommend the CMS to any small or large organization like ours who wants fast answers for day-to-day analysis.
Compact Mass Spectrometry for Fast, Quantitative VOC Screening
Breath analysis is a valuable and widely-used technique that is gaining popularity in medical diagnostics, sports science, substance abuse and other fields where immediate, reliable detection of a variety of in-vivo compounds and their metabolites is required.
However, the actual analysis can be complicated because of the type of data required, the need to sample directly from humans and animals, and the need for real-time access to proximate analytical equipment.
In this recent study by Loughborough University, breath was sampled directly from test subjects into Advion’s expression Compact Mass Spectrometer (CMS). The data collected during this pilot study was very positive and resulted in the go-ahead of a larger study of real-time breath profiling of a much greater population of subjects. Results were superior to those acquired by collection and concentration on TD tubes and then desorbing into large, expensive mass spectrometer systems costing more than 4 times that of Advion’s CMS.
Economic adulteration of milk involves adulteration with nitrogen-rich compounds to interfere with the widely used Kjeldahl protein assay and in ate the total protein content result for economic gains. The economic adulteration issue garnered global attention during the melamine scandal in 2007 which resulted in the sickening and deaths of infants fed with adulterated milk powder. Therefore the ability to rapidly screen milk ingredients in the manufacturing plants for potential economic adulterants is becoming increasingly important.
The current analytical method of choice is based on UPLC-MS/MS which is both expensive and requires considerable expertise for routine use. A simpler and more cost-effective alternative for frontline screening has been developed. The method uses HILIC-based HPLC and single quadrupole mass spectrometry detection to determine the presence of melamine, cyromazin, ammelide and ammeline in milk-based protein commodities.
Register to download this presentation developed by Advion and Abbott Nutrition.
Q: What is the focus of your labs research?
A: Our main research interest is to use mass spectrometry as a routine tool for synthetic intermediates.
Q: What previous workflow challenges did you experience?
A: As we have large numbers of products to run, our major workflow issue is to shorten product detection times.
Q: Why did you incorporate the expression® CMS into your lab?
A: As an open-access instrument, the expression® CMS allows us to incorporate with other instruments with a compact size. More important, it helps us to know the molecular weight information of products timely.
