With the emergence of new protein therapeutics, including biosimilars, biobetters and ADCs, peptide mapping by LC/MS is becoming more commonly used for the identification of post-translational modifications (PTMs), glycosylation, and conjugation sites, as well as primary sequence confirmation.
The purpose of this study is to provide guidelines for developing optimal LC running conditions with Aeris™ PEPTIDE core-shell HPLC/UHPLC columns and determine the feasibility of transferring a method developed on UV to MS simply by adjusting the acidic modifier in the mobile phase.
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: The Drug Discovery Unit (DDU), based at the University of Dundee, is an independent, fully integrated Drug Discovery Group that works across multiple disease areas. We are active in areas of unmet medical need and bridge the gap between academic research and industrial drug discovery. Our staff are multi-disciplinary and come from both Academic and Industrial backgrounds.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: We needed a mass spectrometer that was easy to install, operate and move as well as available at a reasonable price. We have had the instrument for over a year now and are very happy with it.
Q: HOW DO YOU USE THE EXPRESSION CMS?
A: We have the expression linked to an HPLC instrument and it is used as an open access system. The combination of UV purity data along with MS confirmation is incredibly powerful for our chemists. The instrument is networked so our chemists can then process and review data from their desks.
Q: WHAT IS THE FOCUS OF YOUR LAB’S RESEARCH?
A: At SynZeal Research Pvt Ltd, with state of the art synthesis laboratory, we are focused on custom synthesis of high purity secondary reference standards of API Impurities and metabolites. SynZeal also provides chemistry research services and products to synergize the drug discovery and development.
Q: WHAT PREVIOUS WORKFLOW CHALLENGES DID YOU EXPERIENCE?
A: The major challenge to our workflow is to have fast decision on success or failure of targeted chemical transformation to decide further course of action and to complete the projects on time.
Q: WHY DID YOU INCORPORATE THE EXPRESSION CMS INTO YOUR LABORATORY?
A: We selected expression CMS as one of the affordable alternative for Mass Spectroscopy which can be operated even by an inexperienced person in mass spectrometry. Our productivity was increased many fold after installation of this system.
There have been a few reports on thin layer chromatography/mass spectrometry (TLC/MS) and thin layer chromatography/tandem mass spectrometry (TLC/MS/MS). The merits of TLC/MS (i.e., without MS/MS or high-resolution mass measurement) include lower-cost mass spectrometers, ease of operation and molecular weight (MW) information when applicable. TLC/MS/MS offers additional benefits, but requires more expensive instrumentation and more challenging operational requirements.
This article describes the direct coupling of TLC plates or other planar surfaces to a compact, single-quadrupole mass spectrometer that can provide routine electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI) in both the positive and negative ionization modes.
The scientific investigation of cultural heritage objects is the only access to knowledge about long-gone, ancient societies and their cultural, technological as well as their social stage of development. In addition, the materials used by our ancestors, e.g. their textiles and the organic dyestuff to colorize these materials, allow researchers to draw some conclusions about cultural exchanges and trading routes. But in-depth knowledge about materials and dyestuff is also of highest importance for proper conservation and restauration of these materials. Specimens, however, are rare and available only in minute amounts. For their investigation preferentially non-destructive or at least minimal invasive methods have to be used.
A direct analysis using the Atmospheric Solids Analysis Probe (ASAP®) on the expression® compact mass spectrometry (CMS) technique offers a minimally invasive method allowing for a rapid and direct sampling without any sample preparation prior to analysis.
Plate Express™ is a device that provides a simple, automated means of obtaining mass spectra directly from TLC plates, creating a technique known as TLC/MS. Combined with Advion Interchim Scientific’s expression® Compact Mass Spectrometer (CMS) this becomes TLC/CMS. Using this technique chemists can quickly and confidently identify products even in complex mixtures without additional sample preparation.
This application note presents an online thin-layer chromatography (TLC)/CMS technique using the Advion Interchim Scientific expression® compact mass spectrometer (CMS) and Plate Express™ interface to provide compound structural information without sample preparation after TLC separation. A Suzuki reaction for the synthesis of 4-aminobiphenyl will be demonstrated. The online TLC/CMS technique provides rapid and accurate determination of reaction mixture components without the need for off-line TLC sample preparation procedures.
Natural products, the underlying active components and their use in traditional medicine are of increased interest in the field of pharmaceutical research. The naturally occurring active ingredients are ideal chemical starting structures to improve upon in the pharmaceutical drug development process and consequently half of all currently approved drugs in the US are based on natural products. It is therefore not surprising that natural product analysis is a field of major interest world wide. Here, we present a simple example of the added benefits of mass spectrometry in typical natural product analysis workflows based on an alcoholic extract of nutmeg.
The expression compact mass spectrometer (CMS) is a novel high performance, easy-to-use single quadrupole mass spectrometer with enhanced scan speed and the ability for on-line polarity switching and in-source fragmentation.
Analysis of the active components of the cannabis sativa plant is required both for law enforcement where these compounds are illegal and also as a tool for product control and optimization in the increasing number of legal markets for cannabis plant products. Whenever simple, unequivocal and legally defensible analysis methods for the detection and quantification of plant metabolites are required, mass spectrometry is the detector of choice.
Here, we present two simple workflows for the analysis of cannabinoids such as the naturally occuring cannabinol (CBN), the psychoactive ingredient of cannabis sativa, tetrahydrocannabinol (THC) and the degradation product cannabidiol (CBD). Both TLC/FIA/CMS for qualitative detection of cannabinoids, as well as HPLC/CMS for the quantitative determination of THC demonstrate the added benefit of compact mass spectrometry in the analysis of natural products.
With decades of mass spectrometry and chemistry expertise, Advion Interchim Scientific has produced a family of compact mass spectrometers designed for the chemist. The affordability, small size and ease of use make them ideal for use directly at the chemist’s bench, giving immediate answers and informed decisions instead of waiting.
The Advion Interchim Scientific expression® compact mass spectrometer (CMS) is a fast and easy analytical tool for the organic chemist. Ideal for fast reaction monitoring, the expression® CMS features a single quadrupole that can adapt to multiple ionization sources in seconds, including both ESI and APCI. The mass spectrometer works in a variety of applications, including food and beverage, pharmaceutical, biomedical, peptides and proteins, drug discovery and more. The expression® CMS offers a variety of novel sampling techniques, including:
- Direct mass analysis of TLC plates in 30 seconds at the push of a button with Plate Express™
- One-touch analysis of solids and liquid samples with the ASAP® probe
- LC/CMS
- Many more options and configurations to suit your needs
Learn more about the different sample techniques available with the expression® CMS, including fast assay methods for liquids, solids, gases, and even air-sensitive compounds.
Fill out the form to download the full expression® CMS brochure now.
